摘要
背景:低强度脉冲超声波主要用于促进骨折愈合及提高新生骨组织密度,尚未见其对Ⅱ度根分叉病变组织学修复效应的报道。目的:观察低强度脉冲超声波对Ⅱ度根分叉病变的组织学修复效应。方法:5只Beagle犬各取2颗下颌第4前磨牙颊侧作为实验区,建立Ⅱ度根分叉病变模型,分别分为超声组和对照组并在颊侧缺损龈方根面制备切迹作为参照点,高糖饲养8周。超声组行根面平整术,术后1周行低强度脉冲超声波(90mW/cm21.5MHz,200μs,1kHz)处理20min/d,对照组行根面平整术和假处理。6周后取实验区域组织制备脱钙切片,苏木精伊红染色、Masson染色行根分叉区域组织测量分析。结果与结论:超声组根分叉区新生牙槽骨沿根面生长,根分叉区可见牙龈上皮;对照组根分叉区以结缔组织生长为主,可见大量牙龈上皮和少量新生骨组织。超声组新生牙槽骨胶原呈"红-蓝"相间,以红染为主;对照组呈现蓝染,即超声组的新生牙槽骨胶原较对照组成熟。超声组牙槽骨、牙骨质、牙周膜新生量均大于对照组(P<0.05)。提示90mW/cm2低强度脉冲超声波可促进根分叉病变新生牙槽骨胶原成熟和改建。
BACKGROUND：There were many reports on low-intensity pulsed ultrasound（LIPUS） to promote fracture healing and increase bone density,there were not literature involved in tissue repair effects of LIPUS on class Ⅱ furcation involvement.OBJECTIVE：To investigate the tissue repair effects of LIPUS treatment on class Ⅱ furcation involvement.METHOD：Beagle dogs＇ class Ⅱ furcation involvement models were established at buccal region of the dogs＇ fourth premolar of bilateral mandibular and randomly divided into ultrasonic treatment group（UT group） and control group（C group） and set notch at the gingival side of buccal root surface as the reference point.Then,Beagle dogs were fed with high glucose for 8 weeks.Scaling and planning of the root surface was applied on UT group and 1 week later take LIPUS（90 mW/cm2,1.5 MHz,200 μs,1 kHz） to irradiate UT group by 20 minutes per day,while scaling and planning of the root surface and sham irradiation were applied on C group.After 6 weeks,decalcified tissue sections of hematoxylin-eosin staining and Masson staining were used to measure and analysis the furcation tissues.RESULTS AND CONCLUSION：In the UT group,new alveolar bone of furcation area grew along the root surface and gingival epithelium appeared;in the C group,it mainly changed by connective tissue growth at furcation area as well as a large number of gingival epithelium and a small amount of new alveolar bone.The color of new alveolar bone collagen in the UT group was between red and blue,the color of new alveolar bone collagen in the C group showed blue,which meant the new alveolar bone collagen in the UT group was more mature than it in the C group.The new volumes of alveolar bone,cementum and periodontal ligament in the UT group were more than those in the C group（P0.05）.So 90 mW/cm2 LIPUS can promote new bone collagen maturation and remodeling at furcation area.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第7期1219-1223,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(30870754)
重庆市发展和改革委员会高技术产业重大产业技术开发项目(渝发改技[2007]1110号)
重庆卫生局科研项目(2008-2-254)~~
