摘要
目的探讨IgG抗体亲和力指数(AI)在儿童巨细胞病毒(HCMV)感染诊断中的意义。方法分别收集120例临床疑似HCMV感染患儿的尿液和外周血标本,采用实时荧光定量聚合酶链反应(FQ-PCR)和微粒子酶免化学发光法检测尿液HCMV DNA载量和血清HCMV IgM/IgG抗体;采用尿素变性结合酶联免疫吸附试验(ELISA)测定HCMV IgG抗体亲和力指数(AI)。结果 120例疑似HCMV感染患儿的尿液HCMV DNA、血清HCMV IgM和HCMV IgG阳性检出率分别为70.83%(85/120)、60.83%(73/120)和76.67%(92/120),差异有统计学意义(χ2=7.252,P=0.027);25例HCMV IgM-/IgG+患儿中,以高亲和力抗体检出为主,占92%(23/25);67例HCMV IgM+/IgG+患儿中,以低、中等亲和力抗体为主,分别占26.87%(18/67)和53.73%(36/67);高、中、低IgG抗体AI患儿尿液HCMV DNA阳性检出率分别为36.11%(13/36)、86.84%(33/38)和100%(18/18),差异有统计学意义(χ2=32.262,P<0.01)。结论 IgG抗体亲和力指数测定能准确地反映HCMV感染的活动状况,有助于HCMV感染的诊断与治疗。
Objective To investigate the diagnotic significance of the human cytomegalovirus （HCMV） specific IgG antibody avidity index （AI） in children with HCMV infection. Methods Urine and peripheral blood specimens of 120 clinical suspected cases with HC-MV infection were selected respectively. The urine HCMV DNA load and serum HCMV IgM/IgG of which were detected by fluorescent quantitative PCR （FQ -PCR） and microparticle enzyme chemiluminescence immunoassay. HCMV IgG avidity indexes were measured by urea degeneration combining enzyme - linked immunosorbent assay （ELISA）. Results The positive detection rate of HCMV DNA in urine, serum HCMV IgM and HCMV IgG in 120 patients with suspected HCMV infection were 70.83% （85/120）, 60.83% （73/120）and 76.67% （92/120） , and there was significant difference （X2 = 7. 252, P = 0. 027 ）. In 25 cases of HCMV IgM -/IgG ＋ children, high avidity antibodiess accounted for 92% （23/25） . In 67 cases of HCMV IgM ＋/IgG ＋ children, the low and moderate avidity antibodes accounted for 26.87% （ 18/67 ） and 53.73% （36/67））. The positive rate of urine HCMV DNA in high, moderate and low antibodies AI of children was 36.11% （13/36）, 86.84% （33/38） and 100% （ 18/18 ） respectively, and there was significant difference （X2 = 32. 262, P 〈 0. 01 ）. Conclusion Determination of IgG antibody AI can accurately reflect the activities of HCMV infection status,which is helpful for diagnosis and treatment of HCMV infection.
出处
《医学研究杂志》
2011年第3期55-57,共3页
Journal of Medical Research
基金
浙江省人口与计划生育科研基金资助项目(2009031)
