摘要
目的:建立测定人血浆中帕瑞昔布的方法。方法:采用反相高效液相色谱法,血浆以无水乙醚提取后进样测定,色谱柱为KromasilC18,流动相为乙腈-水(92:8),内标为布洛芬,流速为1.0mL·min-1,检测波长为209nm,最小进样间隔为30min。结果:帕瑞昔布血药浓度在73.69～442.16μg·mL-1范围内线性关系良好(r=0.9992);方法回收率为97.26%～99.24%,提取回收率为79.27%～80.51%,日内和日间RSD均≤2.10%。结论:本方法简便、快速、准确、重复性好,可以满足帕瑞昔布血药浓度的测定要求。
OBJECTIVE：To develop a method for the concentration determination of parecoxib in human plasma.METHODS：RP-HPLC method was adopted.The plasma sample was extracted with absolute ether.The determination was performed on Kromasil-C18（250 nm×4.6 nm,5 μm）column with mobile phase consisted of acetontrile-water （92：8） at the flow rate of 1.0 mL·min-1.Ibuproren （IBF） was used as internal standard and UV detection wavelength was set at 209 nm.Minimum injection interval was 30 min.RESULTS：The linear range of parecoxib was 73.69～442.16 μg·mL-1（r=0.999 2）.The method recovery was 97.26%～99.24% and extraction recovery was 79.27%～80.51%.The RSD of intra-day and inter-day were less than 2.10%.CONCLUSION：The method is simple,rapid,accurate,reproducible and suitable for the determination of parecoxib concentration in human plasma.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第18期1675-1677,共3页
China Pharmacy
