摘要
从实验室保存菌种中筛选出一株具有诱导大豆抗阿特拉津的青霉菌株snef960。利用正交试验优化了发酵培养基组分,其最佳碳氮比为葡萄糖(4%)、硝酸铵(0.6%),发酵培养基中4种无机盐的最佳配比为:氯化钾(0.04%)、磷酸氢二钾(0.15%)、硫酸镁(0.04%)、硫酸亚铁(0.001%)。通过单因素试验确定青霉snef960的最佳摇瓶发酵条件:培养基初始pH值为8,发酵时间为9 d,转速为180 r·min-1,100 mL的三角瓶装液量为70 mL,接种量为10%。
Snef 960 which have efficiency to induce anti-atrazine in soybean was screened from laboratory save strains.Cultivation medium of snef960 was researched by orthogonal experiment.The result showed that the optimal C/N ratio was composed of 4% glucose,0.6% NH4NO3,the optimal inorganic salts ratio was composed of 0.04% KCl,0.15% K2HPO4,0.04% MgSO4·7H2O,0.001% FeSO4.The culture conditions were screened with mono-factor test,and the most suitable technical parameters were summed up as follows： pH8 before sterilized,fementation time 9 d,agitation 180 r·min^-1,volume 70/100mL,inoculated volume 10%.
出处
《大豆科学》
CAS
CSCD
北大核心
2011年第2期272-276,共5页
Soybean Science
基金
现代农业产业技术体系岗位科学家专项资助项目
关键词
大豆
阿特拉津
真菌
筛选
发酵条件
Soybean
Atrazine
Fungi
Screening
Fermentation conditions
