摘要
目的:观察结扎肝动脉对胆道梗阻大鼠肝脏的影响并探讨其损伤的机制。方法:将63只SD大鼠按照随机化原则分为A组:结扎胆总管0h、3d、7d,各组动物(n=7);B组:结扎胆总管3d、7d后恢复胆肠内引流伴结扎肝动脉,每组动物(n=21)。各组术后又分为1d、3d、7d三个时段。观察术后大鼠的病死率,ALT、ALP、TBIL、HGF的变化,及肝脏的病理改变。结果:梗阻性黄疸大鼠ALT、ALP、TBIL、HGF均明显升高,各时相与CBDL0h手术对照组比较均有统计学意义(P<0.05)。(IBD+HAL)术后7d时,CBDL7d组HGF浓度与CBDL3d组比较有统计学差异(P<0.05)。HGF与ALT呈明显的正相关性(P<0.05)。结论:HGF增高可能与梗阻性黄疸大鼠的预后不良有关。胆汁郁积性肝损害严重时不宜结扎肝动脉。
Objective To investigate the impact on liver injury after hepatic artery ligation in jaundiced rats,and discuss the mechanism.Methods Sixty-three Sprague-Dawley rats were randomized into A group and B group.A group included CBDL 0h,3d,7d（each group n=7）.B group included CBDL 3d,7d（each group n=21）,and then internal biliary drainage with hepatic artery ligation（IBD＋HAL） in animals of B group were performed,respectively.The mortality rate of rats and the pathological change of the liver were observed,serum ALT,ALP,TBIL and HGF levels were detected following the（IBD＋HAL）.Results Compared with CBDL 0h control group,there were significant difference of serum ALT,ALP,TBIL and HGF levels after the biliary obstruction（P0.05）.The serum HGF levels of CBDL 7d group were evidently higher than CBDL 3d group at 7d（P0.05）,there was positive correlation between levels of HGF and serum ALT（P0.05）.Conclusion The higher HGF levels may be related to a poor outcome in jaundiced rats.The hepatic artery ligation was not recommended in rats with severe cholestatic liver injury.
出处
《放射免疫学杂志》
CAS
2011年第2期191-193,共3页
Journal of Radioimmanology
关键词
梗阻性黄疸
结扎肝动脉
胆肠内引流
肝功能
肝细胞生长因子
obstructive jaundice
hepatic artery ligation
internal biliary drainage
liver function
hepatocyte growth factor（HGF）
