摘要
跨损伤DNA合成(translesion DNA synthesis,TLS)是细胞应答DNA损伤时的一种耐受机制,它利用特异的低保真度的DNA聚合酶直接在损伤的对面合成DNA,使复制得以延续.TLS分为无错旁路和易错旁路两种途径,其中易错旁路途径是DNA损伤诱发基因组突变的主要机制.另外TLS也与肿瘤细胞耐药性相关.在体内执行TLS功能的DNA聚合酶主要是DNA聚合酶Y家族的成员,其中包括聚合酶kappa(Polκ).就TLS的特性,哺乳动物Polκ的结构及催化活性、表达及调控、蛋白质相互作用及其在TLS中可能的调控机制和体内功能等方面做一阐述.
Tanslesion DNA synthesis （TLS） is one mode of DNA damage tolerance in cells upon genotoxic agent treatments, which utilizes specialized low-fidelity DNA polymerases to traverse replication-blocking lesions. TLS can be classified into two categories： error-prone TLS and error-free TLS. Error-prone TLS is one of the fundamental mechanisms for genome mutagenesis. In addition, recent studies suggest that TLS is closely related to tumor chemoresistance. So far, multiple TLS polymerases have been identified. The known major TLS polymerases belong to Y-family DNA polymerases, which include PolK. The general properties of TLS and the current understanding of Polk in mammals were summarized.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2011年第3期204-209,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目(30970588,30970931)~~
