摘要
为构建含筛选基因并能在转染细胞内建立HBV复制状态的真核表达载体，利用BamHI与BglH粘端互补的特点，将经BamHI线性化的HBVDNA（adr亚型）克隆于表达新霉素基因的载体pcDNA3的BamHI与BglII位点间，获得含头尾串联双拷贝HBV基因组的重组质粒。
Here we report the process of constrUcting the eukareotic expression vector which contains screening gene and canestablish HBV replication statUs. The vector pcDNA3 which can express neomycin gene in transfected cells was used in thisexperiment. HBV DNA (adr subtype) linearized by Burn m from pADR-l was inserted betWeen the Burn HI and Bgl 11 sites ofpcDNA3, and the recombinant plasmid PADR-l containing one copy of HBV genome was obtained firstly. After PADR-l waslinearized by Burn HI and ligase with another HBV DNA molecule, we got a recombinant containing tWo copies of HBV genome.Restriction analysis showed that the tWo HBV DNA molecules in this recombinant are linked in head-to-tail fashion. Thisrecombinant was named PADR-2.
出处
《第一军医大学学报》
CSCD
1999年第3期266-268,共3页
Journal of First Military Medical University
