摘要
目的:探讨姜黄素体外对氧化应激致肝星状细胞(HSC-T6)增殖、表达转化生长因子-β1(TGF-β1)的抑制作用。方法:以100μmol.L-1过氧化氢(H2O2)致HSC氧应激,将细胞分成对照组、H2O2组和H2O2+姜素素干预组(终质量浓度分别为25,50,100μmol.L-1)5组,用四甲基偶氮唑(MTT)比色法检测HSC细胞增殖;用流式细胞术检测姜黄素对HSC细胞周期的影响;硫代巴比妥酸法、黄嘌呤氧化酶法和消化法分别检测细胞培养液丙二醛(MDA)、超氧化物歧化酶(SOD)和羟脯氨酸(HyP)的含量;用逆转录聚合酶链式反应(RT-PCR)检测HSC表达TGF-β1的水平。结果:HSC经H2O2诱导后,细胞增殖显著(P<0.01),细胞培养液上清中MDA,Hyp含量明显上升,SOD活力下降(P<0.01);经姜黄素各剂量干预后,MDA,Hyp含量下降,SOD活力升高,以50,100μmol.L-1姜黄素浓度作用明显(P<0.01);H2O2刺激HSC后,TGF-β1 mRNA表达被上调(P<0.001),经姜黄素干预后,TGF-β1 mRNA表达水平下降(P<0.01)。结论:姜黄素可显著抑制氧化应激致HSC的增殖,该作用可能与其抗氧化、抑制TGF-β1的表达有关,表明姜黄素作为抗氧化剂在肝纤维化防治中具有重要价值。
Objective：To observe the inhibitory effect of curcumin on hepatic stellate cell（HSC）-T6 cell proliferation and transforming growth factor β1（TGF-β1） mRNA expression induced by oxidative stress.Method： The proliferation of HSC-T6 cell was examined by MTT colorimetry.The methods of thiobarbituric acid,xanthine oxidase and digestion were respectively applied to determine the levels of malondialdehyde（MDA）,superoxide dismutase（SOD） and hydroxy proline（HyP） of culture mediums.The mRNA expression of TGF-β1 by HSC was determined by RT-PCR.Result： HSC cell proliferation was remarkably increased in the presence of H2O2（P 0.01）,the content of MDA and HyP of HSC in culture mediums was remarkably increased,and SOD activity was decreased（P 0.01）.Curcumin could concentration-dependently inhibit HSC-T6 cell proliferation.After the intervention of different content curcumin,the content of MDA and HyP was remarkably decreased,SOD activity was increased,especially for those treated by 50,100 μmol.L-1of curcumin（P 0.01）.Moreover,TGF-β1 mRNAexpression of HSC was remarkably increased induced by H2O2（P 0.001）.After the intervention of different concentration of curcumin,TGF-β1 mRNA expression level was decreased（P 0.01）.Conclusion： All the results indicated curcumin could remarkably inhibit HSC-T6 cell proliferation and TGF-β1 mRNA expression induced by oxidative stress suggesting that curcumin,as an antioxidative stress factor,may have a potential therapeutic value for liver fibrosis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第9期203-206,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省中医药局项目(2009276)
广东食品药品职业学院院级项目(2007012)
关键词
姜黄素
肝星状细胞
氧化应激
转化生长因子Β1
curcumin
hepatic stellate cells
oxidative stress
transforming growth factor beta 1
