摘要
目的：探讨ERβ在结肠癌细胞株Lovo、HT-29的表达和他莫昔（tamoxifen,TAM）对结肠癌细胞株抑制作用.方法：体外培养人结肠癌细胞株Lovo、HT-29,用细胞爬片免疫组化、RT-PCR、Western blot方法检测ER α、ERβ表达,用MTT法检测不同浓度的17β-E2、TAM、5-Fu、TAM＋5-Fu、TAM对结肠癌细胞株的生长影响作用.流式细胞仪（FCM）测定细胞凋亡率.结果：①在结肠癌细胞株Lovo、HT29中稳定表达ERβ而不表达ER α ②TAM、5-Fu能抑制Lovo、HT29细胞株的增殖 ③TAM协同5-FU对Lovo、HT29细胞株有促使细胞凋亡的作用.结论：①结肠癌细胞株Lovo表达ERβ而不表达ER α ②TAM以ERβ为靶点抑制Lovo细胞株的增殖 ③TAM协同5-FU抑制Lovo细胞株的增殖,TAM协同5-FU对Lovo、HT29细胞株促细胞凋亡的作用.
Objective： To evaluate the expression of ERβ in human colon cancer cell lines Lovo, HT-29 and the effect of tamoxifen （TAM） on their apoptosis and growth. Methods： The expression of ERα, ERβ was determined by immunohistochemistry, RT-PCR and Western blot method. The effects of different concentration of 17β-E2, TAM ,5-FU, TAM ＋ 5-FU, TAM on human colon cancer cell lines Lovo, HT-29 were evaluated by using MTT. Apoptosis of the cells was measured by FCM method. The difference among different arms was analyzed by One-way ANOVA analysis. Results ：The ERβ could be detected in Lovo,HT-29 cells, while the ERa could not. The Lovo, HT-29 cells growth could be inhibited by TAM and 5-FU. IC50： Lovo： 3.81 ＋0.34 μmol/L,TAM 15.52 ±1. 33 μmoL/L; H-29： 5-FU 4.11 ±0.63 μmoL/L,TAM 16.23 ＋ 2.85 μmol/L. Two cells were also treated with 17β-E2.There was no difference between 17β-E2 and control group （P 〉0.05）. The apoptosis rates of Lovo,HT-29 were increased when they were treated by TAM. The TAM combined 5-FU could amplify the effect of 5-FU. Conclusion ： ERβ is expressed in Lovo, HT-29 cells,but ERα is not expressed. TAM can inhibit Lovo, HT-29 cells growth, and the results showed a dosedependent relation in vitro. The mechanism of inhibition may be due to apoptosis induced by TAM. The mechanism of apoptosis induced by TAM is due to inhibition of ER expression. The TAM combined 5- FU can synergy the effect of 5-FU.
出处
《广州医学院学报》
2010年第4期15-18,共4页
Academic Journal of Guangzhou Medical College
