摘要
背景:低钾右旋糖苷液用于肺移植,灌注保存效果较好,但有效供肺保存时间仍较短,价格较昂贵。目的:观察改良自制低钾右旋糖苷液保存供肺不同时间供肺变化,评价改良自制低钾右旋糖苷液的供肺保存期限。方法:成年家犬32只,随机分成对照、实验两组。对照组采用低钾右旋糖苷液作为供肺灌注保存液,实验组采用改良自制低钾右旋糖苷液。比较两组供肺组织灌注前、灌注后、保存4,8,12,24h的髓过氧化物酶、丙二醛、湿干比、病理光镜检查、透射电镜检查结果。结果与结论:两组离体肺组织的髓过氧化物酶、丙二醛和湿干比值随着保存时间的延长呈渐增趋势,髓过氧化物酶值在保存12h以后与灌注前有显著差异,丙二醛和湿干比值保存8h后与灌注前有显著差异。保存8h后,实验组肺组织结构和超微结构的变化仍然可逆,较对照组变轻,但保存4,12,及24h两组变化基本相似。结果提示,在实验条件下,供肺在改良自制低钾右旋糖苷液中保存8h后开始出现显著变化,保存12h后开始出现不可逆变化。
BACKGROUND：Clinical application of low potassium dextran（LPD） solution has better effectiveness,but effective period of donor lung preservation is still short and price is expensive.OBJECTIVE：To observe changes of lung preservation after different periods preserved with modified self-confected LPD solution and to evaluate the storage term of lung in this solution.METHODS：In total 32 healthy dogs were randomly divided into the control group and experimental group.Dogs in the control group were perfused with LPD solution;and those in the experimental group were perfused with modified self-confected LPD solution.Changes of myeloperoxidase,malonaldehyde,wet to dry weight ratio（W/D）,pathological result and super-micro structural results from donor lung tissues were compared at pre-perfusion,after perfusion,and 4-,8-,12-,as well as 24-hours of preservation.RESULTS AND CONCLUSION：For both groups：the values of myeloperoxidase,malonaldehyde and W/D were increasing as lung preservative period in vivo was prolonging;after 12-hour preservation,the value of myeloperoxidase was significant changed while the value of malonaldehyde and W/D were changed significantly after 8-hour preservation;the pathological and super-micro structural changes were reversible till lung preservative period reached 8-hour.Changes of lung preservation started to be significant after 8-hour preserved with modified self-confected LPD solution and irreversible historical changes appeared after 12-hour preservation.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第53期9881-9887,共7页
Journal of Clinical Rehabilitative Tissue Engineering Research
