摘要
目的检测心脉隆注射液中的蛋白质含量。方法运用Bradford法检测心脉隆注射液蛋白质的含量,并结合二阶导数光谱-标准加入法排除干扰。结果利用二阶导数光谱-标准加入法建立了一个测量蛋白质浓度的通用标准曲线,并以此为基础检测到心脉隆注射液中存在蛋白质,含量约为0.15 mg·mL-1。结论二阶导数光谱-标准加入法可以准确测定心脉隆注射液中蛋白质含量。
OBJECTIVE To evaluate the protein content in Xinmailong injection.METHODS The protein content in Xinmailong injection was measured by Bradford assay connected with standard addition method of second derivative spectrum method to exclude interference.RESULTS The present study was conducted to elucidate that the protein content of about 0.15 mg·mL-1 present in Xinmailong injection and a common standard curve to detect the content present in protein by standard addition methods of second derivative spectrum method was established.CONCLUSION The protein content in Xinmailong injection could be evaluate accurately by the method of Bradford assay connected with standard addition method of second derivative spectrum method.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第4期338-341,共4页
Chinese Journal of Modern Applied Pharmacy
基金
云南省教育厅(06Y287C)
云南省重点项目(2008IF012)
大理学院博士启动基金
关键词
心脉隆注射液
标准加入法
二阶导数光谱
蛋白质含量
Xinmailong injection
standard addition method
second derivative spectrum method
protein content
