摘要
[目的]建立西藏天麻(RHIZOMA GASTRODIAE)多糖含量的测定方法。[方法]采用苯酚-浓硫酸比色法进行测定。[结果]以葡萄糖为标准品,苯酚-硫酸法测定天麻多糖含量的最佳反应条件为:6%苯酚溶液1 ml,浓硫酸5 ml,水浴温度25℃。西藏天麻多糖以葡萄糖(C6H12O6)计,在21%～33%之间,平均值为28%,多糖的含量高,具有较高的使用价值和经济价值。[结论]该研究中的测定方法简便、准确,可用于评价西藏波密天麻药材的质量。西藏天麻多糖的含量较高,是一种大有潜质的保健食品和药材,开发及利用西藏天麻,对带动当地经济有非常重要的意义。
[Objective] To determine the content of polysaccharide in Tibetan RHIZOMA GASTRODIAE.[Method] Phenol-sulfuric acid colorimetric method was adopted.[Results] Glucose as standard reference,the optimum conditions for the determination of polysaccharide in Tibetan RHIZOMA GASTRODIAE were as follows：1 ml,6% phenol,5 ml sulfuric acid,water bath temperature of 25 ℃.The contents of C6H12O6 in RHIZOMA GASTRODIAE were in the range of 21%-33%,and the average value was 28%,which proved Tibetan RHIZOMA GASTRODIAE was of high nutritional and economic values.[Conclusion] The method adopted in this study was accurate and simple,which was suitable for evaluating the quality of Tibetan RHIZOMA GASTRODIAE.
出处
《安徽农业科学》
CAS
北大核心
2011年第8期4564-4566,4573,共4页
Journal of Anhui Agricultural Sciences
关键词
西藏天麻
苯酚-浓硫酸比色法
多糖含量
测定
Tibetan RHIZOMA GASTRODIAE
Phenol-sulfuric acid colorimetric method
Polysaccharide content
Determination
