摘要
ＩＬ２Ｒα、β、γｍＲＮＡ上起始密码子ＡＵＧ的下游序列， 合成与之互补的硫代反义寡聚脱氧核糖核酸（ＩＬ２Ｒα、β、γＳＯＤＮｓ） ， 长度为２０ｎｔ。实验结果表明，ＩＬ２ＲαＳＯＤＮｓ 对外周血淋巴细胞生长最高抑制率可达到８１－９２％ ； 而ＩＬ２ＲβＳＯＤＮｓ和ＩＬ２ＲγＳＯＤＮｓ 的抑制作用较低， 其最高抑制率均为５９－１ ％ 。在１０μｍｏｌ／ＬＩＬ２ＲαＳＯＤＮｓ 作用后的第４８ 小时，ｓＩＬ２Ｒ 的表达下降８０％ ； ｍＩＬ２Ｒα的表达下降５１－５％ 。
Inhibition of IL 2R expression and proliferation of the human peripheral blood lymphocytes in vitro was achieved by saturating the cells with synthetic 20nt antisense phosphorothioated oligodeoxynucleotides(S ODNs) complementary to the AUG initiation codon of the mRNA encoding the human IL 2Rα,β,γ The maximal inhibition rates of the lymphocytes proliferation were 81 92%,59 1% and 59 1% with regard to the action of IL 2Rα,β,γ S ODNs respectively After 48 hours by the action of 10μmol/L IL 2Rα S ODNs,the inhibition of the sIL 2R (soluble) reached the maximal effect(80%),and the expression of the mIL 2R(CD25) on the cell surface was decreased by 51 5%.
出处
《上海免疫学杂志》
CSCD
北大核心
1999年第5期267-270,共4页
Shanghai Journal of Immunology
关键词
IL-2R
外周血淋巴细胞
反义S-ODNs
表达
IL 2R
peripheral blood lymphocyte
antisense phosphorothioated oligodeoxynucleotides
