摘要
目的分析拟诊莱姆病患者血清学检查结果,为有效诊治莱姆病提供科学依据。方法应用间接免疫荧光试验(IFA)、ELISA和蛋白免疫印迹(WB)3种实验方法,对此例患者进行血清抗伯氏疏螺旋体IgM和IgG抗体检查,并结合临床疾患进行综合分析。结果 3种试验方法IgG均为阳性,其中IFA的IgG抗体滴度达到1:128,WB的IgG有2条蛋白带P83和P39;3种试验方法IgM均为阴性。结论患者临床发展病程符合莱姆病的特征,实验室结果阳性,莱姆病的诊断可以成立。
Objective To analyze the serological results derived from suspected Lyme Disease patients for efficient diagnosis and treatment.Methods Using the indirect immunofluorescence assay（IFA）,enzyme-linked immune sorbent assay（ELISA） and western blot（WB）,suspected Lyme Disease patients were tested for Borrelia burgdorferi IgM and IgG antibodies.A comprehensive analysis was conducted in combination with clinical cases.Results IgG antibodies were positive as shown by IFA,ELISA and WB methods.The titer of IgG antibodies was up to 1：128 as shown in IFA and two protein bands,P83 and P39,were present in WB.IgM antibodies were negative as shown in the three test approaches.Conclusion Based on the typical clinical course and positive laboratory results of the patients,the diagnosis of Lyme disease can be confirmed.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2011年第2期175-177,共3页
Chinese Journal of Vector Biology and Control
关键词
莱姆病
血清学
间接免疫荧光试验
酶联免疫吸附试验
Lyme disease
Serology
Indirect immunofluorescence assay test
Enzyme-linked immune sorbent assay
