摘要
目的 观察ＩＬ１ 对成纤维细胞生长的影响及其作用机理，为从细胞因子角度开展抗纤维化研究提供依据。方法 用ＭＴＴ比色、ＡｇＮＯＲＳ 银染和免疫组化技术，观察正常和受照ＮＩＨ／３Ｔ３ 成纤维细胞（６０Ｃｏγ源一次照射，吸收剂量３０ Ｇｙ）生长增殖活性、ＡｇＮＯＲＳ 和ＤＮＡ合成以及Ⅲ型胶原表达。结果 ＩＬ１ 浓度为１００ ×１０３Ｕ／Ｌ，培养第３ 天时，成纤维细胞数量和增殖活性（Ａ 值） 为对照值的１－４ 和１－３ 倍（照射组Ａ值为对照的１－１ 倍）；ＡｇＮＯＲＳ 颗粒数和Ｓ期细胞比例分别升高至对照值的２－９ 和２－０ 倍；而Ⅲ型胶原表达阳性细胞数为对照值的１－９ 倍。结论 在一定浓度范围内，ＩＬ１ 能促进成纤维细胞的增殖活性，ＤＮＡ 合成和Ⅲ型胶原表达。
Objective\ Effects of interleukin\|1(IL\|1) on proliferation of fibroblasts and their mechanism were analysed in order to provide experimental basis for studying antifibrotic activity from the point of cytokines. Methods MTT colorimetry,a silver colloid techniqiue of AgNOR\-S,and immuno\|histochemical methods of anti\|type Ⅲ collagen and anti\|bromodeoxyuridine(BrdUrd) were used to observe the proliferative activity,AgNOR\-S and DNA synthesis and expession of type Ⅲ collagen in normal and irradiatd (30 Gy single irradiation by\+\{60\}Co γ\|rays) fibroblasts. Results When IL\|1 of 100×10\+3 U/L was used,the counts and optical density(A) value of normal fibroblasts were 1\^4 and 1\^3 times higher than those of control group respectively, at the third day after culturing.At the same time,the mean number of AgNOR\-S and percentage of normal fibroblasts in S\|phase were 2\^9 and 2\^0 times higher than those of control group,while the number of type Ⅲ collagen positive fibroblats increased by 1\^9 times. Conclusion In a definite range of concentration,IL\|1 could promote proliferative activity,AgNOR\-S and DNA synthesis and expression of type Ⅲ collagen of fibroblasts.\;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1999年第5期334-336,共3页
Chinese Journal of Radiological Medicine and Protection
关键词
IL-1
成纤维细胞
MTT
AGNORS
Ⅲ型胶原
Interleukin\|1\ \ Fibroblasts\ \ Gamma radiation\ \ Type Ⅲ collagen\ \ DNA synthesis
