摘要
目的了解安徽皖北地区临床分离的耐甲氧西林葡萄球菌(MRS)所携带的7种耐药基因状况。方法使用聚合酶链反应(PCR)检测197株MRS中mecA、gyrA、qacA/B/C、qacA、erm/A/B/C、ermBt、etM耐药基因。结果 197株MRS中,mecA基因携带率为100.0%,gyrAt、etM、qacA/B/C、qacA、ermA/B/C、ermB基因携带率分别为:58.9%、48.7%、45.7%、35.5%、29.4%、16.2%;其中耐甲氧西林金黄色葡萄球菌(MRSA)gyrAt、etM、ermA/B/C、ermB基因携带率高于耐甲氧西林凝固酶阴性葡萄球菌(MRCNS),而qacA/B/C、qacA基因携带率低于MRCNS,差异有统计学意义(P<0.01)。结论安徽皖北地区MRS中mecA、qacA/B/C、qacA基因携带与国内报道类似,而gyrA、TetM、ermA/B/C、ermB低于相关报道。
OBJECTIVE To investigate the seven kinds of resistance gene status in Northern Anhui in methicillin-resistant Staphylococcus aureus（MRS）.METHODS A total of 197 MRS in mecA,gyrA,qacA/B/C,qacA,ermA/B/C,ermB,TetM resistance gene were detected by using polymerase chain reaction（PCR）.RESULTS Of 197 MRS,the carrier rate of mecA gene was 100.0%,gyrA,TetM,qacA/B/C,qacA,ermA/B/C,ermB gene were 58.9%,48.7%,45.7%,35.5%,26.4% and 16.2% and the carrier rates of gyrA,tetM,ermA/B/C,ermB genes of methicillin-resistant S.aureus（MRSA）were higher than MRCNS（methicillin-resistant coagulase-negative Staphylococci）,but the carrier rates of qacA/B/C,qacA genes of（MRSA） were lower than MRCNS（P0.01）.CONCLUSION The situation of MRS carrying mecA,qacA/B/C or qacA gene is as same as the domestic report,while the gyrA,TetM,ermA/B/C or ermB is lower than the relevant reports.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第8期1507-1510,共4页
Chinese Journal of Nosocomiology
基金
安徽省淮北市科技局自然科学资金资助(080244)
关键词
耐甲氧西林葡萄球菌
基因
皖北
Methicillin-resistant Staphylococcus
Genes
Northern Anhui
