摘要
为评价添加中草药成分的卷烟中活性成分向烟气中的转移率,建立了测定卷烟及其烟气中的岩白菜素,黄芩苷,甘草苷,丹皮酚,五味子素,罗汉果苷等六种成分含量的高效液相色谱法.方法：HypersilC18-ODS 5μm（200×4.6 mm）;流动相：A∶B=0.2%磷酸水∶乙腈,梯度洗脱;检测波长：205 nm;进样量：10μL;流速：1 mL/min.结果：6种中草药成分在一定范围内线性关系良好,相关系数均大于0.998,RSD都小于4.0%（n=6）,加标回收率在97.6%~100.3%之间.结论：六种成分中岩白菜素,丹皮酚及五味子素的转移率较高：分别为7.37%、15.26%、9.30%.本方法操作简单,精密度好,准确度高,可用于卷烟及烟气中六种成分的测定.
To evaluate transfer rate of active ingredient of Chinese herbs from the purpose-made cigarette to its smoke,a new high performance liquid chromatography（HPLC） method was developed for determinating the six compounds in the cigarette and its smoke simultaneously.Six analytes were Bergenin,Baicalin,Glycyrrhizin,Paeonol,Schisandrin,Mangosteen and Glycosides,respectively.Methods： Hypersil C18-ODS 5 μm（200×4.6 mm）;mobile phase：A=0.2% aqueous phosphoric acid,B=acetonitrile;gradient elution;detection wavelength：205 nm;injection volume：10 μL;flow rate：1 mL/min.Results： All six herbal ingredients had a good linear relationship in a certain range,correlation coefficients were greater than 0.998,the relative standard deviation were less than 4.0%（n=6）,recovery rates were in the range of 97.6%~100.3%.Conclusions： Among the six components,Bergenin,Paeonol and Schisandrin have higher transfer rate：Bergenin 7.37%,Paeonol 15.26%,Schisandrin 9.30%.The proposed method was found to be simple,precise and accurate and it is suitable for the simultaneous determination of six active components in the cigarette and its smoke.
出处
《湖南师范大学自然科学学报》
CAS
北大核心
2011年第1期60-64,共5页
Journal of Natural Science of Hunan Normal University
基金
国家自然科学基金资助项目(20927005
20875028
20080542003
2008[890])
国家"863"课题(2010AA023001)
湖南省科技计划资助项目(05k009)
