摘要
从基因库中调取已完成测序的昆虫化学感受蛋白(chemosensory proteins,CSPs)基因序列和氨基酸序列,共涉及昆虫7个目23个种的59个基因。应用MEGA 5.0形成了这59个基因的系统发育关系树,并计算CSPs基因在昆虫中的核苷酸替换速率。对非同义替换速率和同义替换速率进行比较的结果显示,CSPs基因在昆虫中总体上受到负选择作用。用PAML软件位点-特异模型检测到6个受到正选择作用的位点,其中5个位于功能重要的区域。
The phylogenetic trees of the insect chemosensory proteins（CSPs） gene from 59 sequences of CSPs in 23 species under 7 orders published in GeneBank were generated by MEGA 5.0.Complete comparisons of CSPs sequences were used to determine the rates of synonymous（Ks） and nonsynonymous（Kn） codon substitutions and the ratio of Kn/Ks was calculated to estimate of the selection pressure on CSPs genes in insects.The Kn/Ks ratio was far less than 1,suggesting that CSPs may be evolving under negative selection.By the site-specific model in PAML 4.4,6 amino acid sites undergoing positive selection were detected,5 of them were in the important function regions.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第2期177-181,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31071713)
教育部高等学校博士学科点专项科研基金(20094404110019)
关键词
昆虫
化学感受蛋白
基因
进化
insect
chemosensory proteins
gene
evolution
