摘要
目的比较心肌内移植内皮祖细胞(EPCs)和注射碱性成纤维生长因子(bFGF)对扩张型心肌病大鼠心功能改善的影响。方法 50只雌性SD大鼠皮下注射异丙肾上腺素,制作扩张型心肌病模型。4周后,36只模型鼠随机分为EPCs组,bFGF组和对照组,每组12只。3个月后,心脏彩色超声评估心功能情况;彩色微球技术评估局部心肌血流量(RMFM);Y染色体原位杂交示踪移植的EPCs;采用逆转录PCR分析bFGF mRNA的表达,ELISA分析心肌组织内bFGF的分泌情况。结果细胞移植3个月后,EPCs组大鼠心肌内存在sry基因阳性细胞,这些细胞融合在新生的或原有的血管壁内。心脏彩色超声发现,EPCs组和bFGF组大鼠的心功能改善,RMFM提高,在EPCs组尤为明显。EPCs组心肌内血管密度、bFGF mRNA的表达和分泌均明显高于bFGF组和对照组(P<0.05)。结论心肌内移植EPCs是安全可行的治疗扩张型心肌病的方法。心功能的改善与血管新生、RMFM增加密切相关。EPCs移植与单次心肌内注射bFGF比较,疗效更好。
Objective To compare the different effects of endothelial progenitor cells（EPCs） transplantation and basic fibroblast growth factor（bFGF） intramyocardial infusion on cardiac function of dilated cardiomyopathy（DCM） rats. Methods Fifty adult female rats received subcutaneous in- jection of isoprenaline for induction of DCM. Four weeks later,the model rats were randomly di- vided into EPCs group,hFGF group and control group of 1Z rats each. Three months later , echocardiographic examination and regional myocardial blood flow（RMFM） measurement were per- formed. EPCs were traced by fluorescence in situ hybridization. The protein and mRNA expression of hFGF in each group was measured by ELISA assay and reverse transcription-polymerase chain reaction respectively. Results Three months after intramyocardial transplantation of EPCs, Sry gene positive cells were detected only in EPCs group,these cells were present in walls of new and original vessels. The cardiac function as well as RMFM were significantly improved in EPCs group and bFGF group,the improvement was more significant in EPCs group. There was higher capillary density,expression of protein and mRNA of bFGF in EPCs group compared with bFGF group and control group （P〈0.05）. Conclusions Transplantation of EPCs in myocardium can improve cardiac function,induce neovascularization and increase RMFM in DCM rats. The treatment with EPCs has better efficacy than administration of bFGF alone.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2011年第4期357-360,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
