摘要
目的 制备低免疫原性靶向微泡.方法 采用不同膜磷脂成份制备表面包埋或暴露生物素的靶向微泡（MBb或MBe）,荧光鉴定＂生物素-链亲和素＂桥连技术构建靶向微泡的可靠性.以普通微泡（MBc）为参照,酶联免疫吸附法体外检测三组微泡与人血清反应后的补体3a（C3a）水平;平行平板流动腔实验检测各组微泡与包被有链亲和素培养皿的黏附力.结果 MBe及MBb均发出明亮的环形绿色荧光,而MBc未见荧光.MBb组的C3a水平[（1.037±0.047）ng/m1]明显低于MBe组[（1.326±0.042）ng/m1]（P＜0.05）,与MBc组[（1.004±0.031）ng/m1]差异无统计学意义（P＞0.05）.平行平板流动腔实验显示MBb组黏附的微泡数[（15.2±11.3）个/视野]少于MBe组[（103.2±28.3）个/视野]（P＜0.05）,与MBc组[（17.8±11.9）个/视野]差异无统计学意义（P＞0.05）.结论 成功制备屏蔽表面免疫原性物质的靶向微泡,为进一步活体实验提供了基础数据.
Objective To prepare targeted micorbubble with low immunogenicity. Methods The microbubbles were produced with different phospholipids and identified by the fluorescent method. Detect the level of C3a after reaction with human serum in vitro with enzyme-linked immunosorboent assay （ELISA） method and the number of microbubble binding with the streptavidin packed on the dish by using the parallel plate flow chamber. Results The level of C3a was （1.037±0.047）ng/ml in MBb group,（1. 326 ± 0. 042）ng/ml in MBe group and （ 1.004 ± 0.031 ） ng/ml in MBc group. The level of C3a in MBb group was significantly lower than that in MBe group（ P ＜0.05）,and there was no significantly difference between MBb group and MBc group （ P ＞ 0. 05）. The parallel plate flow experiments showed that the number of MBb（15.2 ± 11.3） in each field of view binding with the streptavidin packed on the dish was significantly fewer than that of MBe （ 103.2 ± 28.3） （ P＜0.05 ）, and there was no significantly difference between MBb and MBc（17.8 ± 11.9） （ P ＞0.05）. Conclusions The targeted microbubble with low immunogenicity has been prepared successfully,which can be used for further experiment in vivo.
出处
《中华超声影像学杂志》
CSCD
北大核心
2010年第12期1079-1081,共3页
Chinese Journal of Ultrasonography
基金
国家自然基金（30870716,30770567）
关键词
微气泡
免疫活性
生物学标记
Microbubbles
Immunocompetence
Biological markers
