摘要
目的：检测环境富集对孕期应激子代海马磷酸化的环磷酸腺苷反应元件结合蛋白（p-CREB）表达的影响.方法：将SD孕鼠随机分为2组,一组动物不做任何处理,其子代断乳后在标准条件下喂养,为正常组;另一组动物在孕第13～19天接受限制性应激,其子代断乳后,一半在标准条件下喂养,构成应激组,另一半在子代环境富集条件下喂养（构成应激＋环境富集组）.子代在生后35d,用免疫组织化学和免疫印迹方法分别检测海马p-CREB的表达.结果：p-CREB阳性产物广泛表达于海马各区神经元.免疫印迹方法显示应激组p-CREB表达量明显少于正常组;应激＋环境富集组海马p-CREB的表达量也明显多于应激组,与正常组的差别无统计学意义.结论：环境富集能有效逆转孕期应激导致的子代海马p-CREB表达下调.
Objective： To detect the effects of enriched enviroment on the hippocampal phosphorylated cyclic adenosine mono-phosphate response element binding protein （p-CREB） of prenatal stressed offsprings. Methods： Pregnant SD rats were ran- domly divided into two groups. One group received no stress. Their offsprings named as the control group were raised at the standard environment. The other group received restraint stress on gestation day 13-19. After wearing at postnatal day 21, half of the offspring named as the stress-benriched environment group were raised at the enriched environment on postnatal day 22-35, and the others forming the stressed group were given the standard environment. The p-CREB expression in the hippoeampus was detected by immunohistochemistry staining and Western blot. Results： Compared to the normal group, the p-CREB expression of the hippocampus in the stressed group was significantly decreased. The p-CREB expression of the hip- poeampus in the stress＋enriched environment group was significantly higher than that of the stressed group. Conclusion： Enriched environment can efficiently reverse the downregulation of hippocampal p-CREB expression in offsprings induced by prenatal stress.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2010年第6期768-770,共3页
Chinese Journal of Anatomy
关键词
孕期应激
环境富集
海马
环磷酸腺苷反应元件结合蛋白
子代
大鼠
prenatal stress
enriched environment
hippoeampus
p-cyclic adenosine monophosphate response element binding protein
offsprings
rat
