摘要
目的:利用体外细胞试验评价羟基喜树碱纳米晶体hydroxycamptothecin nanocrystals,HCPT-NCs)抗肿瘤作用及其机制。方法:以羟基喜树碱注射液(hydroxycamptothecin injection,HCPT-I)对肝癌SMMC-7721细胞的干预作用作为对照,应用MTT法检测羟基喜树碱纳米晶体对肝癌细胞SMMC-7721细胞的增殖抑制作用,流式细胞术分析细胞凋亡率,RT-PCR方法检测survivin基因表达的变化。结果:与对照制剂HCPT-I相比,HCPT-NCs对SMMC-7721肿瘤细胞生长抑制和杀伤作用,促凋亡能力和对survivin基因靶向作用,有显著性差异(p 0.05)。结论:HCPT-NCs对SMMC-772细胞的生长有较明显的抑制作用,促进SMMC-7721细胞凋亡可能和下调survivin的表达有关,和HCPT-I相比作用明显。
Objective： Investigate the effect and mechanism of HCPT nanocrystal induced cancer using cell experiment in vitro.Methods ：Compared to HCPT injection,anti-proliferative effect of HCPT nanocrystal against SMMC-7721 cell was tested by MTT assay.Measured the cell apoptosis rates by treatment with different concentration of HCPT nanocrystal by the method of flow cytometry;RT-PCR technologies was applied to assessing the expression of survivin.Results： Compared to HCPT injection,with the inducing SMMC-7721 cell apoptosis and reducing the expression of survivin,HCPT nanocrystal showed significantly difference.Conclusion： HCPT nanocrystal showed the significant effect on inhibiting the cell proliferation of SMMC-7721 and induced SMMC-7721 cell apoptosis,may be it associated with reducing the expression of survivin.Compared to HCPT injection,HCPT nanocrystal＇s effect is better.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2010年第6期28-30,共3页
Pharmacology and Clinics of Chinese Materia Medica
