摘要
使用气升式光生物反应器培养钝顶螺旋藻25天,发现随螺旋藻生物量的增长,细菌生物量有一定的增长趋势。应用PCR-DGGE技术分析螺旋藻培养过程中细菌种类组成,发现在不同时期细菌群落组成变化明显。通过DGGE图谱中18条特征条带的克隆、测序及系统进化分析,发现其中有8条序列与α变形菌纲序列相似,3条与拟杆菌纲序列相似,2条与γ变形菌纲序列相似,2条与异常球菌纲序列相似,2条与放线菌纲相似,还有1条与蓝藻纲的序列相似。在所有相似序列中,有17条来源于高温、碱性、淡水环境,与已报道的螺旋藻的培养环境相似。研究发现α变形菌纲与拟杆菌纲的细菌为整个培养过程中的优势类群。
Using airlift reactor to cultivate Spirulina plarensis for 25 days, bacterial biomass increased with the increase of the biomass of S. plarensis. The bacterial community composition of different cultural periods changed significantly. Eighteen DGGE bands were excised, cloned and sequenced. Among these sequences, 8 were similar to α-Proteobaeteria, 3 were similar to Bacteroidetes, two were similar to γ- Proteobacteria, two were similar to Deinococci, two were similar to Actinobacteria, and 1 was similar to Cyanobacteria. There were 17 sequences rooted in the similar environment of the S. plarensis culture. During the culture, α-Proteobacteria and Bacteroidetes were the superior genera.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2011年第1期148-156,共9页
Oceanologia Et Limnologia Sinica
基金
国家"863"项目资助
2007AA09Z434号
国家908专项
908-01-ZH3号
中国科学院创新项目
KZCX2-YW-213-3
