摘要
目的:探讨慢性铅暴露儿童的机体氧化应激水平。方法:采用石墨炉原子吸收光谱法,检测贵屿镇118名3～7岁儿童血铅浓度;ELISA免疫酶联吸附法检测尿液中8-羟基脱氧鸟苷(8-OHdG)水平。结果:贵屿镇儿童血铅均值为(8.2±3.5)μg/dL,将血铅水平按四分位数分成4组进行统计学处理,与0.0～5.9μg/dL组尿中8-OHdG水平比,5.9～7.4与9.4～21.6μg/dL组明显增高(P<0.05)。结论:慢性铅暴露儿童尿液中8-OHdG水平的升高,表明儿童机体处于较高的氧化应激状态,对儿童生长发育会造成一定的伤害。
Objective： To investigate the oxidative stress level in children with chronic lead exposure.Methods： The blood and urine samples were collected from 118 children in Guiyu town,the blood lead level was determined by graphite furnace atomic absorption spectrometry.The urine analysis for 8-hydroxy-2′-deoxyguanosine（8-OHdG）was performed by commercial ELISA kit.Results： The blood lead level of 118 children was（8.2±3.5）μg/dL.According to the blood lead level,the study group was divided by quartile.Compared with 0.0～5.9 μg/dL group,a significant increase of 8-OHdG level in urine was observed in 5.9～7.4 and 9.4～21.6 μg/dL groups.Conclusion： Lead exposure can affect 8-OHdG level in urine in children,suggesting an increase of oxidative stress level in children with a higher blood lead level which might be harmful for children＇s development.
出处
《汕头大学医学院学报》
2011年第1期32-34,共3页
Journal of Shantou University Medical College
基金
国家自然基金资助课题(30972513)
高等学校博士学科点专项科研基金资助项目(200805600004)
