摘要
通过气相色谱分析幼虫信息素的10种酯类在中蜂和意蜂成年工蜂的分布情况。将成熟的封盖子脾提至人工培养箱中孵化,幼蜂出房时对其进行标记,再放回原群中。取刚出房幼蜂、10日龄和20日龄这三种工蜂的头部、胸部和腹部样品,用于气相分析。结果表明,中华蜜蜂和意大利蜜蜂工蜂个体主要含有甲基亚油酸酯(ML)、乙基亚油酸酯(EL)、甲基亚麻酸酯(MN)和乙基亚麻酸酯(EN),这些酯类在工蜂各节分布情况不一。
Ten esters found in brood were studied in adult workers of Apis mellifera ligustica and Apis cerana cerana. Newly emerged workers were obtained from frames of sealed brood placed in an incubator. One-day-old bees from the same colony were marked on the dorsal surface of the thorax with a spot of paint. Newly emerged bees, nurses (10-day-old) and foragers (20-day-old) bees were collected. body sections of workers were extracted, derivatized, and analyzed for MP, MN, MS, MO, ML, EP, EL, EN, EO, ES. The results showed that body sections of different age worker in Apis cerana cerana and Apis mellifera ligustica are both dominated with MP, EP, MN and EN. The content of these esters on the body sections is different.
出处
《中国农学通报》
CSCD
北大核心
2011年第5期409-411,共3页
Chinese Agricultural Science Bulletin
基金
江西省教育厅科学研究项目"中华蜜蜂表皮信息素成分鉴定及其应用"(GJJ09163)
关键词
幼虫信息素
工蜂
气相色谱分析
brood pheromone
workers
gas chromatography ( GC )