摘要
分子标记基因分型误差对研究结果的影响越来越为研究者们所关注。本研究以茄子(Solanum melongenaL.)野生和栽培类群为研究材料,从微卫星荧光基因分型中PCR扩增实验体系和荧光标记判读2个环节控制实验的误差率,总结出一套简便、快速的微卫星分子标记PCR扩增实验优化流程;并且,针对微卫星荧光标记基因分型中存在的各种误差,提出相应的分析方法和检测方案,为利用微卫星分子标记技术的研究提供实验操作依据和数据处理参考。
More and more researchers focus on genotyping errors because of their effects on the biological conclusions of a study. Using the fluorescent microsatellite genotyping of wild and cultivated eggplants（Solanum melongena L.） as example,error rates had been limited from these two processes that were PCR（polymerase chain reaction） amplification and identification of the fluorescence peak in this study. The rapid and simple protocol was created for optimizing PCR amplification conditions. And some strategies were proposed to discern and assess errors from the fluorescence data. Our study provided some reference for experiment operation and data handling of microsatellite markers.
出处
《中国农学通报》
CSCD
北大核心
2011年第6期117-123,共7页
Chinese Agricultural Science Bulletin
基金
科技部国家重点基础研究发展计划973"人工选择和基因组进化"(2007CB815700)
国家自然科学基金青年基金项目"芍药组的分子系统发育和物种形成"(3090008601)
关键词
基因分型误差
茄子
荧光微卫星基因分型
genotyping errors
Solanum melongena L.
fluorescent microsatellite genotyping
