摘要
选用国家长沙苎麻圃的790份种质资源,在已有的25个性状数据的基础上,采取优先取样+多次聚类变异度取样方法,利用离差平方和法的聚类方法,无序质量性状采用simplematching,有序质量性状和数量性状采用欧氏距离,在20%的抽样水平下,构建了158份苎麻核心种质。构建的核心种质的质量性状多样性指数均值、数量性状方差差异百分率、变异系数变化率均高于原种质,极差符合率100%;质量性状的性状频率分布与原种质有差异的性状为12.5%;原种质的各数量性状间的相关性共适应基因系统基本上在核心种质中得到保留。构建的核心种质可以很好的代表原种质的遗传多样性。
Based on 25 agronomic traits from 790 ramie germplasm of National Ramie Germplasm Nursery in Changsha, the 158 core collections were developed by Ward＇s clustering method, stepwise clustering with deviation sampling strategy and stepwise clustering with preferred sampling strategy, using simple matching in disordered qualitative characters and Euclidean distance in ordered qualitative characters and quantitative characters at 20% proportion. Mean of genetic diversity, variance difference percentage and variable rate of variation coefficient in core collection were higher than initial collection; coincidence rate of range was 100% in core collection. Frequency distribution of 12.5% qualitative characters in core collection was different from initial collection. The core collection could not only conserve the pattern and quantity of each trait, but also keep the genetic diversities of initial collection.
出处
《中国麻业科学》
2011年第2期59-64,共6页
Plant Fiber Sciences in China
基金
农业部作物种质资源保护项目(NB09-2130135-41
NB2010-2130135-30(2))
国家科技基础条件平台项目(2005DKA21002-10)
中国农业科学院科技经费项目资助
关键词
苎麻
核心种质
抽样方法
聚类方法
遗传距离
ramie
core collection
sampling method
clustering method
genetic distance
