摘要
目的探讨胃癌中血管内皮生长因子C(VEGF-C)和黏蛋白1(MUC1)的表达与胃癌侵袭和淋巴结转移的关系。方法采用SP免疫组化技术,分别检测207例胃癌组织、癌旁组织、区域淋巴结及40例正常胃黏膜组织中VEGF-C及MUC1的表达水平。同时应用间接免疫荧光法检测VEGF-C和MUC1在正常胃黏膜细胞系(GES-1)、胃高分化腺癌细胞系(N87)、胃低分化腺癌细胞系(BGC-823)的表达和定位。结果 VEGF-C和MUC1在胃癌组织中的阳性表达率明显高于正常胃黏膜及癌旁组织;在区域淋巴结癌转移组中的阳性表达率明显高于无癌转移组(P<0.01);伴有淋巴结转移的胃癌组织中VEGF-C,MUC1的阳性表达率明显高于无淋巴结转移组织(P均<0.01);VEGF-C及MUC1表达与淋巴结转移、浸润深度、TNM分期及分化程度有密切关系(P均<0.01),而与患者年龄、性别、肿瘤大小无关(P均>0.05)。VEGF-C及MUC1在正常胃黏膜细胞系、高分化胃癌细胞系、低分化胃癌细胞系的表达有显著性差异(P均<0.01)。结论 VEGF-C和MUC1的表达可作为评估胃癌患者转移与复发的一个观测指标。
Objective It is to investigate the relationship between the expression of vascular endothelial growth factor-C（VEGF-C） and Mucin 1（MUC1） in gastric carcinoma and the invasion and metastasis of tumor. Methods Streptavidin peroxidase immunohistochemistry technique（SP） was used to detect the expression of VEGF-C and MUC1 in 207 gastric carcinoma specimens,207 specimens of gastric mucosa adjacent to carcinoma,207 specimens of regional lymph node and 40 specimens of normal gastric mucosa.Indirect immunofluorescence was used to investigate the expression and location of MUC1 and VEGF-C proteins in gastric cancer cell lines of various differentiations. Results The positive expression rates of VEGF-C and MUC1 in gastric carcinoma were markedly higher than that in normal gastric mucosa and gastric mucosa adjacent to carcinoma.The positive expression rates of VEGF-C and MUC1 in metastatic regional lymph nodes were significantly higher than that in non metastatic regional lymph nodes（P〈0.01）.The positive expression rates of VEGF-C and MUC1 in gastric carcinoma specimens in patients with lymphatic metastasis were significantly higher than that in without lymphatic metastasis（P〈0.01）.The positive expression levels of VEGF-C and MUC1 were significantly correlated with lymph node metastasis,tumor infiltration depth,TNM staging and gastric cancer differentiated extent（P〈0.01）,but not with age,sex and the size of gastric cancer（P〉0.05）.There were significantly different in the expression of MUC1 and VEGF-C among normal epithelial cell of gastric mucosa,Well-differentiated adenocarcinoma cell of gaster and Poor-differentiated adenocarcinoma cell of gaster（P〈0.01）. Conclusion The expressions of VEGF-C and MUC1 can be used as a reliable indicator to evaluate the metastasis and recurrence of gastric cancer.
出处
《现代中西医结合杂志》
CAS
2011年第12期1434-1437,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词
胃癌
血管内皮生长因子C
黏蛋白1
gastric cancer
vascular endothelial growth factor-C
Mucin 1
