摘要
目的:研究抗环瓜氨酸肽(抗CCP抗体)、抗角蛋白抗体(抗AKA抗体)和类风湿因子(RF)的联合检测在类风湿关节炎(RA)诊疗中的临床价值。方法:选取2009年1月—2010年11月我院门诊及住院患者210例,其中RA组110例,其他风湿性疾病组65例,健康对照组35例,所有患者均检测抗CCP抗体、抗AKA抗体和RF,分别用酶联免疫吸附试验(ELISA)、间接免疫荧光法、乳胶凝集法检测。结果:抗CCP抗体、抗AKA抗体和RF对RA诊断的灵敏度分别为72.7%、42.7%、82.7%,抗CCP抗体与RF灵敏度之间差异无显著性,RF及抗CCP抗体均明显高于抗AKA抗体;3种抗体对RA诊断的特异度分别为95%、99%、77%,抗CCP抗体、抗AKA抗体的特异度均比RF高。对RA的诊断联合3种抗体均阳性特异性为99%,灵敏度为40%;3种抗体中的任2项阳性的特异度为100%,灵敏度为33.6%;1种抗体阳性的特异度为54%,灵敏度为26.4%,3种抗体均阳性与1种抗体阳性的灵敏度及特异度的差异均具有统计学意义。结论:在目前应用的3种特异性抗体中,抗CCP抗体对RA的诊断价值最高,联合检测抗CCP抗体、抗AKA抗体和RF可以提高RA的诊断价值。
Objective：To study the clinical significance of anti-cyclic citmllinase peptide antibody（anti-CCP）,antikeratin antibody（AKA）and rheumatoid factors（RF）in diagnosing rheumatoid arthritis（RA）.Methods：From Jan 2009 to Nov 2010,210 cases including the 110 cases with rheumatoid arthritis,65 cases with others rheumatoid disease and 35 cases of healthy control were performed the test of anti-CCP with enzyme-linked immunosorbent assay（ELISA）,test of AKA with indirect immunofluorescence technique and the test of RF by latex aggregation method.Results：The diagnosis sensitivity of ACCP,AKA and RF for RA were 72.7%,42.7% and 82.7% respectively,While the diagnosis specificity of three antibody were 95%,99% and 77% respectively.The difference of sensitivity between CCP and RF were no significant difference,and the sensitivity of RF and ACCP were higher than that of AKA.The specificity of ACCP and AKA were higher than that of RF.The diagnosis specificity of RF with three antibodies positive was 99% while the sensitivity was 40%.The specificity of RF with two of three antibodies positive was 100% while the sensitivity was 33.6%.The specificity of RF with one of three antibodies positive was 54% while the sensitivity was 26.4%.There were significant higher in the sensitivity and specificity of three antibodies positive than that of two of three antibodies positivity in diagnosis of RA.Conclusion：ACCP has higher value in diagnosis of RA in three specific anti-bodies.Joint detection of ACCP,AKA and RF can improve the diagnosis specificity of RA.
出处
《医学理论与实践》
2011年第6期629-631,共3页
The Journal of Medical Theory and Practice
关键词
抗CCP抗体
抗AKA抗体
类风湿因子
类风湿关节炎
Anti-cyclic citmllinase peptide antibody
Antikeratin antibody
Rheumatoid factors
Rheumatoid arthritis
