摘要
目的 评价未损伤背根神经节（L4背根神经节）7氨基丁酸A受体（GABAA受体）在大鼠神经病理性痛中的作用。方法成年雌性SD大鼠30只，体重200～250g，随机分为3组（n=10）：对照组（C组）、蝇蕈醇组（M组）和荷包牡丹碱组（B组）。采用结扎L脊神经的方法制备大鼠神经病理性痛模型。c组于k背根神经节局部注射生理盐水50出；M组于k背根神经节局部注射GABA。受体激动剂蝇蕈醇50μl；B组于k背根神经节局部注射GABA。受体拮抗剂荷包牡丹碱50弘l；各组注射时间均大于1min。于术前1d至术后10d，每天测定大鼠的热痛阈和机械痛阈。结果与c组比较，M组热痛阈差异无统计学意义（P〉0．05），机械痛阈升高，B组热痛阈和机械痛闽均降低（P〈0．05）。结论未损伤背根神经节GABA。受体激活参与了神经病理性痛大鼠机械痛敏的发生发展，对热痛敏的发生发展可能不起主导作用。
Objective To evaluate the role of GABAA receptors in uninjured dorsal root ganglion （L4 DRG） in a rat model of neuropathic pain. Methods Thirty adult female SD rats weighing 200-250 g were randomly divided into 3 groups （n = 10 each）： control group （group C）, muscimol group （group M） and bieuculline group （group B） . Neuropathic pain was produced by L5 spinal nerve ligation. Normal saline 50 μl, GABAA receptor agonist-muscimol 50 μl or GABAA receptor antagonist- bicucullin 50μl was injected into the L4 DRG. The thermal pain threshold and mechanical pain threshold were measured and recorded from 1 day before operation to 10 days after operation. Results Compared with group C, the mechanical pain threshold was significantly increased （ P 〈 0.05 ）, while no significant difference was found in thermal pain threshold in group M （ P 〉 0.05 ）, and the thermal pain threshold and mechanical pain threshold were significantly decreased in group B （P 〈 0.05） . Conclusion Activation of GABAA receptors in uninjured DRG is involved in mechanical hyperalgesia in a rat model of neuropathic pain, but it dose not play a leading role.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第12期1443-1445,共3页
Chinese Journal of Anesthesiology
基金
十堰市科学研究与开发计划项目（2009s06）
