摘要
目的探讨组织型纤溶酶原激活物（t—PA）对ECV304细胞血管内皮细胞生长因子（VEGF）表达的影响及过程中可能涉及的信号通路。方法将ECV304细胞与Adt—PA混合培养24h后检测VEGF转录及表达水平并与混合培养48h组及空病毒组作比较。检测空病毒转染组及转基因组ECV304细胞中磷酸化ERK（pERK）水平，以及应用PD98059阻断ERK磷酸化后细胞内VEGF的表达变化。结果ECV304细胞内VEGF转录和表达水平在24h和48h两个时问点较对照组均有明显升高（P〈0．01）。转染后细胞内pERK水平呈逐渐升高的趋势，而加入PD98059的ECV304细胞中VEGF蛋白的表达量及pERK水平明显下降（P〈0．01）。结论外源性t-PA可显著增加ECV304细胞中VEGF的转录和蛋白表达水平。在此过程中，ERK信号途径起重要作用。
Objective To investigate the expression of vascular endothelial growth factor （VEGF） in ECV304 cells after transfection with Ad tissue plasminogen activator （t-PA） in vitro and the signaling pathway involved. Methods The expression of VEGF in ECV304 cells was detected after mixed culture with Adt-PA for 24 h as group 1, and compared with that in group 2 （mixed culture with Adt-PA for 48 h） and group 3 （mixed culture with Ad for 48 h）. The expression of phosphorylated extracellular regulated protein kinases （pERK） and the expression changes of VEGF after blocking phosphorylation of ERK by PD98059 were examined. Results The expression of VEGF was increased obviously in groups 1 and 2 as compared with group 3 （P 〈 0. 01 ）. After transfection with Adt-PA, ERK could be activated obviously in ECV304. PD98059 could decrease the expression of VEGF and pERK in ECV304 cells （P 〈 0. 01 ）. Condusion Exogenous t-PA can promote VEGF mRNA transcription and protein expression in ECV304 cells. ERK signa- ling pathway plays an important role during this up-regulation process.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第4期496-498,共3页
Chinese Journal of Experimental Surgery
基金
基金项目：苏州市科技局社会发展科技计划资助项目（SS0727）
