摘要
目的:探讨表皮生长因子(Epidermal growth factor,EGF)和口腔鳞癌侵袭转移的关系。方法:采用ELISA和免疫组化法测量不同浓度EGF作用于口腔鳞癌TSCCa细胞系及颈淋巴转移癌GNM细胞系后血管内皮生长因子(vascular endothelial growth factor,VEGF)活性和阳性表达变化;同时采用凝胶电泳迁移实验(EMSA)检测不同浓度EGF下核转录因子-κB(nuclear factor kappa B,NF-κB)的活性变化。结果:不同浓度的EGF(0ng/mL,10ng/mL,40ng/mL,80ng/mL)作用于两种细胞系后,随着外源性EGF浓度的增加,GNM和TSCCa细胞中VEGF活性和阳性表达有增加,NF-κB的活化明显增强,VEGF和NF-κB的活性与EGF有剂量依赖关系,与对照组相比差异有显著性(P<0.05);而在相同的浓度内TSCCa细胞较GNM细胞VEGF和NF-κB的活性增加较明显,两者相比差异有显著性(P<0.05)。结论:EGF可能是通过增加VEGF的活性和阳性表达,调节NF-κB的信号传导路径来促进OSCC细胞侵袭和转移的。
Objective：To detect the role of epidermal growth factor （EGF）on the invasiveness and metastasis of human oral squamous cell carcinoma.Method：Using ELISA and immunhistiochemistry detect the activity and expression of VEGF in oral squamous cell carcinoma cell lines TSCCa and metastasis carcinoma cell lines GNM treated by different levels of EGF.The activity of nuclear factor kappa B（NF-κB） was determined by electrophoretic mobility shift assay（EMSA）.Result：The different levels of EGF（0 ng/mL,10 ng/mL,40 ng/mL,80 ng/mL） had increased the expression and activity of VEGF and NF-κB in TSCCa and GNM cell lines,Moreover,the activity of VEGF and NF-κB had more dependent on EGF,which had higher significent compared to the control group（P 0.05）.However,the activity of VEGF and NF-κB in TSCCa cell lines was increased higher than that of in GNM cell lines after the same concentrations of EGF treatment.Conclusion：EGF may be promoted the invasion and metastasis potential of oral squamous cell carcinoma,and enhanced of the expression and activity of VEGF and upregulated the activity of NF-κB in this process.
出处
《临床口腔医学杂志》
2011年第3期133-135,共3页
Journal of Clinical Stomatology
关键词
表皮生长因子
口腔鳞癌
肿瘤转移
血管内皮生长因子
核转录因子-KB
epidermal growth factor
oral carcinoma
tumor metastasis
vascular endothelial growth factor
nuclear factor kappa B
