摘要
背景:透明质酸是一种用途广泛的高分子材料,在实际应用中,由于其相对分子质量较大,透皮吸收效果不理想,利用脂质体作为其载体可以改善其传输效果,具有较好的应用前景。目的:观察包裹低相对分子质量透明质酸脂质体的透皮性能。方法:采用薄膜法制备低相对分子质量透明质酸脂质体,设计正交实验,采用蛇蜕研究其透皮吸收性能。结果与结论:温度35℃、胆固醇与卵磷脂的比例为0.15∶1,透明质酸与卵磷脂的比例为0.03∶1、水合介质PBS的pH值为7.5,可制备包封率较高的低相对分子质量透明质酸脂质体;脂质体包裹低相对分子质量透明质酸,能有效得提高透明质酸透过蛇蜕的能力,达到了提高透明质酸透皮吸收率的作用。
BACKGROUND： Hyaluronic acid is a versatile polymer material, in practical applications, due to its large molecular weight, percutaneous absorption effect is not satisfied, the use of liposomes as a carrier can improve its transmission effect, with a good application prospect. OBJECTIVE： To study the percutaneous penetration of the liposomes carrying low-molecular-weight hyaluronic acid. METHODS： Low-molecular-weight hyaluronan liposomes were prepared through thin films. Orthogonal experiment was design, and percutaneous penetration was study by snake slough. RESULTS AND CONCLUSION： The low-molecular-weight hyaluronan liposomes could enwrap more hyaluronic acid when the temperature is 35 ℃, the ratio of cholesterol and lecithin is 0.15∶1, the ratio of hyaluronic acid and lecithin is 0.03∶1, and the pH of hydration medium PBS is 7.5. Hyaluronic acid could penetrate the snake slough more easily when they were enwrapped by liposome, and the percutaneous penetration of hyaluronic acid was improved.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第3期465-467,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
