摘要
用7种培养基和2种分离方法对稻曲病菌的培养及分离技术进行了研究。结果表明，胁本哲氏培养+抑制细菌生长的氯霉素（5oμg／mL）是分离稻曲病菌最适宜的培养基。稻谷是厚垣孢子产生的理想培养基。厚垣孢子悬液法可用于稻曲病菌的快速分离，而稻曲球不同层次组织块分离法可从保存长时间的稻曲球分离到稻曲病菌。
Effect of the kinds of media, isolation methods and the stored periods of false smut ball on the isolation ofUstilagonoidea virens have been primarily studied to seek ideal isolation technique. The resuIts showed Wakimoto Toceshi+chloromycetin (5oμg /mL) is an ideal medium for the pathogen isolation, and rice grain is the best medium for the sporulationof chlamycladospores so far studied. The methods of chlamycladosporal suspension can be used for the rapid isolation of thepathogen, and the inner tissue can successfully isolate the pathogen from false smut balls which have been stored for a longtime (1o months ).
出处
《中国水稻科学》
CAS
CSCD
北大核心
1999年第3期186-188,共3页
Chinese Journal of Rice Science
关键词
稻曲病菌
分离技术
培养基
菌落
孢子
culture media
colony
isolation techniques
spores
Ustilagonoidea virens
