摘要
目的:通过黑木耳多糖对兔动脉粥样硬化斑块中FAK的表达的影响,探讨黑木耳多糖降血脂和抗氧化以外的抗AS形成机制。方法:将40只健康雄性新西兰白兔随机分为三组:①空白对照组(正常组)8只,喂基础饲料;②阳性对照组(模型组)16只,高脂高胆固醇饲料喂养;③木耳多糖组(预防组)16只,每天每兔给高脂高胆固醇饲料+5克木耳多糖喂养。12周末所有实验用兔麻醉处死,行主动脉粥样硬化病理形态学观察。通过HE染色、免疫组织化学染色对斑块内的病变进行定位、定量分析。结果:FAK在正常组呈弱阳性表达,而在模型组和预防组中血管内膜和中膜呈阳性表达。预防组与模型组对照的结果显示,FAK在预防组中的表达较模型组明显减少。结论:黑木耳多糖可抑制动脉粥样硬化斑块中FAK的表达,从而减少血管平滑肌细胞参与的动脉粥样硬化形成,发挥抗AS形成的作用。
Objective：to investigate the effects of auricularia auricular polysaccharide（AAP） on the expression of FAK in rabbit atherosclerosis（AS） plaques for a better insight to the role of AAP in resolution of AS.Methods： 40 New Zealand white rabbits were randomly divided into 3 groups： normal group（n=8）,model group（n=16）,preventing group（n=16）.Normal group always received normal chow;model group always received cholesterol chow;preventing group always received cholestrol chow and 5g AAP.At the end of twelve week,all rabbits were killed to perform the pathologic examinations.In addition,the number of cell of FAK was analyzed by transmission microscopy and immunohistochemistry.Through HE,collagen changes will be found increasing with the atherosclerotic lesion progression.Results： SABC immunohistochemistry analysis： The expression of the focal adhesion kinase（FAK） can be seen low expression of the FAK in nomal group rabbits.Rabbits of model group showed high expression of the FAK,the number of FAK in rabbits of preventing group decreased significantly.Conclusions ： Auricularia auricular polysaccharide could inhibit the expression of FAK,thus decreased the formation of atherosclerosis.
出处
《牡丹江医学院学报》
2011年第1期1-3,共3页
Journal of Mudanjiang Medical University
基金
牡丹江医学院科研项目(B2006-01)
