摘要
目的构建野生型DPP Ⅳ真核表达质粒。方法将野生型DPP Ⅳ基因定向克隆到真核表达质粒pcDNA3.1(+),构建成重组质粒pcDNA3 DPP Ⅳ;然后用限制性内切酶消化和DNA序列测定鉴定。结果经酶切和DNA序列测定鉴定证实重组质粒构建正确。结论真核表达质粒pcDNA3DPP Ⅳ构建成功,为进一步研究DPP Ⅳ基因在卵巢癌细胞中的表达和生物学作用奠定了基础。
Objective To construct an eukaryotic expression plasmid inserted wild type DPP IV gene. Methods To construct recombinant plasmid pcDNA3.1 DPP IV, the wild type DPP IV gene was cloned into eukaryotic expression plasmid pcDNA3.1 （＋）. The accuracy of the plasmid was then confirmed by restriction enzyme digestion and DNA sequencing. Results Restriction enzyme digestion and DNA sequencing confirmed that the recombinant plasmid had been constructed correctly. Conclusion An eukaryotic expression plasmid peDNA3DPP IV had been constructed successfully, which lays a foundation for the investigation of the expression and biological role of DPP IV gene in ovarian carcinoma.
出处
《中国医药科学》
2011年第5期15-17,共3页
China Medicine And Pharmacy
关键词
DPP
Ⅳ
基因
卵巢肿瘤
真核表达质粒
DPP IV
Gene
Ovarian tumor
Eukaryotic expression plasmid
