摘要
目的:探讨HDAC1的异常表达以及HPV16感染与宫颈癌发病的相关性。方法:用PCR技术及免疫组织化学(SP)方法检测110例慢性宫颈炎组织及宫颈上皮内瘤样变组织和宫颈鳞癌组织中HPV16感染以及HDAC1的表达。结果:在慢性宫颈炎组、CINⅠ组、CINⅡ组、CINⅢ组和宫颈癌组中HPV16的感染率分别为10.0%、36.4%、50.0%、66.7%、75.0%,组间差异有统计学意义(P<0.05);HDAC1阳性表达率分别为0、27.3%、41.7%、54.2%和70.0%,各组间差异有统计学意义(P<0.05)。HPV16感染组的HDAC1阳性表达率高于HPV16未感染组,差异有统计学意义(P<0.05)。结论:HPV16感染可能是通过提高组蛋白去乙酰化酶的水平抑制转录,在宫颈癌的发生、发展中起作用。
Objective：To explore the anomalous expression of HDAC1 and the relationship between the infection of HPV16 and cervical cancer. Methods：110 cases of chronic cervititics,CIN and SCC of the cervix uteri were included in this study.The expressions of HDAC1 protein were detected by immunohistochemistry staining as well as HPV16 DNA detected by PCR. Results：The detection rate of HPV16 DNA was 10.0%,36.4%,50.0%,66.7%,75.0% in chronic cervititics,CINⅠ,CINⅡ,CIN Ⅲ and cervical cancer respectively.The differences were statistically significant（P0.05）.The positive expression rate of HDAC1 was 0,27.3%,41.7%,54.2% and 70.0% in chronic cervititics,CINⅠ,CINⅡ,CIN Ⅲ and cervical cancer,respectively.The differences were statistically significant（P0.05）.The positive expression of HDAC1 in the negative expression of HPV16 was significantly lower than the positive expression of HPV16（P0.05）. Conclusion：The infection of HPV16 plays an important role in the carcinongenesis of SCC,which may be through the way to raise Histone deacetyltransferase level to inhibit the transcriotion.
出处
《中国妇幼保健》
CAS
北大核心
2011年第8期1217-1219,共3页
Maternal and Child Health Care of China
