摘要
目的探讨膜联蛋白V（annexinV）在孕妇外周血及胎盘组织中的表达及其与子痫前期发病的关系。方法选取2007年12月-2009年12月河北医科大学第二医院产科住院的子痫前期孕妇120例，其中早发型轻度子痫前期孕妇30例（早发轻度组），晚发型轻度子痫前期孕妇30例（晚发轻度组），早发型重度子痫前期孕妇30例（早发重度组），晚发型重度子痫前期孕妇30例（晚发重度组）；另选同期健康孕妇30例为健康孕妇组。采用蛋白印迹法和免疫组化方法检测孕妇胎盘组织及外周血中的annexinV蛋白表达水平；采用流式细胞仪检测胎盘滋养细胞的凋亡率；采用逆转录（RT）PCR技术测定各组胎盘组织中annexinV mRNA表达水平；并对各组孕妇外周血中的凝血酶原时间（胛）、活化部分凝血活酶时间（APTT）、纤维蛋白原（FiB）进行检测，并计算国际标准化比率（INR）。结果（1）各组胎盘组织及外周血中annexinV蛋白水平：早发轻度组分别为0．54±0．12及0．62±0．17，早发重度组分别为0．47±0．15及0．56±0．24，晚发轻度组分别为0．74±0．23及1．08±0．32，晚发重度组分别为0．68±0．28及0．72±0．21，健康孕妇组分别为1．73±0．35及1．55±0．27。子痫前期各组胎盘组织及外周血中anilexin V水平均低于健康孕妇组，分别比较，差异均有统计学意义（P〈0．05）。但子痫前期各组间分别比较，差异均无统计学意义（P〉0．05）。（2）滋养细胞早期凋亡率、中晚期凋亡率：早发轻度组分别为3．21％、0．86％，早发重度组分别为5．32％、0．72％，晚发轻度组分别为2．43％、0．63％，晚发重度组分别为4．28％、0．48％，健康孕妇组分别为1．05％、0．59％。子痫前期各组胎盘滋养细胞的早期凋亡率增加，与健康孕妇组比较，差异均有统计学意义（P〈0．05）；但子痫前期各组之间分别比�
Objective To evaluate the expression of annexin V in maternal blood and placenta, and to explore the relationship between annexin V and preeclampsia（PE）. Methods 120 women with PE who delivered babies in the Second Hospital of Hebei Medical University from December 2007 to December 2009 were chosen as study groups. They were classified into four groups： early-onset mild group （n = 30）, early-onset severe group （ n = 30） , late-onset mild group （ n = 30） and late-onset severe group （n = 30）. 30 women without perinatal complications who accepted elective term cesarean section were chosen as control group. Western blot and immunohistochemistry were used to detect the expression and localization of annexin V in placenta and maternal blood. Flow cytometry was employed to detect the apoptosis of cytotrophohlast. AnnexinV mRNA level was determined by reverse transcription （RT）PCR. Prothrombin time （PT）, activated partial thromboplastin time （AFFF） , fibrinogen （FiB）, international normalized ratio （INR） were detected in each group. Results （ 1 ） The expression of annexin V in placenta and maternal blood were ： 0. 54 ±0. 12 and 0.62 ±0. 17 in early-onset mild group; 0.47 ±0. 15 and 0. 56 ±0. 24 in early-onset severe group; 0. 74 ±0. 23 and 1.08 ±0. 32 in late-onset mild group; 0. 68 ±0. 28 and 0. 72 ±0. 21 in late-onset severe group; 1.73 ± 0. 35 and 1.55 ± 0. 27 in control group. They were significantly lower in PE groups than in control group （ P 〈 0. 05 ）. However, there was no significant difference among PE groups （ P 〉 0. 05）. （2） The early apoptosis, late apoptosis percentage of trophoblast cells were： 3.21% , 0. 86% , in early-onset mild group; 5.32% , 0. 72% , in early-onset severe group; 2. 43% , 0.63% , in late-onset mild group; 4. 28%, 0.48% in late-onset severe group; 1.05%, 0. 59%, in control group. Early apoptosis percentage in each group of PE was higher than that in control group （ P 〈 0. 05）. However, there was no signific
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2011年第2期88-93,共6页
Chinese Journal of Obstetrics and Gynecology
