摘要
目的探讨维拉帕米对胆管癌细胞QBC939耐药性的影响。方法用MMT法测定维拉帕米(Verapamil,V)对顺铂(Cisplatin,DDP)的半数抑制剂量及剂量效应曲线的影响。结果顺铂对QBC939细胞的半数抑制剂量(IC50)为335.5μg/L,联用10μg/mL,20μg/mL,40μg/mL维拉帕米时顺铂的半数抑制剂量分别为73.20μg/L,29.12μg/L,17.30μg/L,较单用顺铂明显降低(P<0.05),且呈明显的维拉帕米剂量依赖性(P<0.05)。结论维拉帕米能够改善QBC939对顺铂的耐药性,且与药物浓度相关。
Objective To observe the change of drug resistance of QBC939 cells after treatment with verapamil,a competitive inhibitior of P-glycoprotein.Methods MMT was used to test the change of verapamil on cisplatin dose curves.Results The dose of cisplatin of 50% inhibition was 335.5 μg/L,it decreased to 73.20 μg/L,29.12 μg/L and 17.30 μg/L treated with 10 μg/mL,20 μg/mL and 40 μg/mL verapamil,respectively.Conclusions Verapamil can inhibit cisplatin resistance of QBC939,and the inhibitory effect is related with the concentration of verapamil.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2011年第2期133-135,共3页
China Journal of General Surgery
关键词
胆管肿瘤
维拉帕米
顺铂
多药耐药
Bile Duct Neoplasms
Verapamil
Cisplatin
Multi Drug Resistance
