摘要
目的观察PI3-Kinase抑制剂LY294002对人胆管癌细胞QBC939的作用。方法 MTT法检测LY294002对QBC939细胞的生长抑制作用;流式细胞术检测LY294002诱导的细胞凋亡;免疫印迹法分析LY294002对于PI3-K/Akt信号通路中Akt磷酸化及caspase9的表达作用。结果 LY294002可抑制胆管癌细胞QBC939的增殖,促进其凋亡。Western-blotting显示,LY2940022可抑制Akt磷酸化水平,促进caspase3,9的蛋白表达。结论 LY294002可通过抑制Akt信号通路的活性,上调促凋亡分子caspase3,9的表达,抑制胆管癌细胞QBC939的增殖,诱导其凋亡。
Objective To study the effect of LY294002,an inhibitor of PI3-Kinase,on human cholangiocarcinoma QBC939 cells.Methods The cell proliferation was detected by MTT assays.Apoptosis was analyzed by flow cytometry.The phosphorylation of Akt and expression of caspase9 were assessed by Western-blotting.Results LY294002 inhibited the proliferation and induced apoptosis in human cholangiocarcinoma QBC939 cells in a dose-dependent manner.Furthermore,Western-blotting showed that LY294002 attenuated the phosphorylatioin of Akt and up-regulated the expression of caspase3,9.Conclusions LY294002 up-regulates the expression of caspase3,9 by inhibition of the activity of PI3-K/Akt signaling pathway,induction of apoptosis and decreasing cell proliferation.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2011年第2期129-132,共4页
China Journal of General Surgery
