摘要
目的 观察脂质体携载Ｃ型利钠利尿肽（ＣＮＰ） 对大鼠主动脉血管舒张、平均动脉血压和内皮素（ＥＴ） 刺激主动脉平滑肌细胞增殖的影响。方法 主动脉环灌流测定血管张力；平滑肌细胞培养；［３Ｈ］ＴｄＲ参入测定ＤＮＡ合成；反相蒸发法制备脂质体。结果 脂质体携载ＣＮＰ的舒张主动脉和降低平均动脉压作用显著强于单用ＣＮＰ。ＥＴ刺激平滑肌细胞增殖，使ＶＳＭＣ［３Ｈ］ＴｄＲ 参入量增加１－８ 倍。１０ －８ 、１０－ ７ 、１０－ ６ ｍｏｌ·Ｌ－１ ＣＮＰ可使ＥＴ 刺激［３Ｈ］ＴｄＲ参入量分别下降２％ （ Ｐ＞ ０－０５）， ２１ ％ （ Ｐ＜ ０－０５） 和５５％ （ Ｐ＜０－０１） ，而同样浓度的脂质体携载ＣＮＰ 使ＥＴ 刺激ＶＳＭＣ［３Ｈ］ＴｄＲ参入量分别下降５６％ （ Ｐ＜ ０－０１），５９％ （ Ｐ＜０－０１） 和６６ ％ （ Ｐ＜ ０－０１）， 其作用强于ＣＮＰ。脂质体和ＣＮＰ对［３Ｈ］ＴｄＲ 参入量与对照组相比差异无显著性。结论 脂质体携载ＣＮＰ的舒张血管、降低平均动脉压和抑制ＥＴ
AIM In this study, we observed the effect of liposome encapsulated C type natriuretic peptide (CNP) on aortic relaxation, mean arterial blood pressure and endothelin (ET) induced DNA synthesis in cultured vascular smooth muscle cells (VSMCs) of rat. METHODS Perfusion of arterial rings; rat VSMC culture; DNA synthesis was assayed by [ 3H] TdR incorporation; Liposomes were prepared by reverse evaporation from a mixture of egg yolk phosphadyl choline, cholesterol and DOPC (molar rato7∶2∶1). RESULTS Compared with unencapsulated CNP, encapsulated CNP in liposome relaxed rat aortic rings and decreased mean arterial blood pressure greater. ET enhanced the cultured VSMCs [ 3H] TdR incorporation by 1 8 fold. 10 -8 ,10 -7 ,10 -6 mol·L -1 CNP decreased VSMC [ 3H] TdR incorporation induced by ET 2% ( P >0 05), 21% ( P <0 05) and 55% ( P <0 01), but equivalent concentrations of the encapsulated CNP in liposome significantly reduced VSMC [ 3H] TdR incorporation by 56% ( P< 0 01), 59% ( P <0 01) and 66% ( P <0 01), and unencapsulated CNP and liposomes were without significant effect on the VSMCs [ 3H] TdR incorporation. CONCLUSION The magnitude of the encapsulated CNP in liposome relaxation of aortic rings, reduction of mean arterial blood pressure and inhibition of VSMC proliferation significantly greater compared with equivalent concentration of the unencapsulated peptide
出处
《中国药理学通报》
CAS
CSCD
北大核心
1999年第5期407-407,共1页
Chinese Pharmacological Bulletin
基金
"九五"国家科技攻关项目
