摘要
目的研究硝普钠、7-硝基吲唑(7-NI)和AMT对急性脑缺血后海马神经元细胞是否具有保护作用。方法将80只SD大鼠随机分组,按照不同给药时间和给药方法制作全脑缺血再灌注模型,待全脑缺血15 min后再灌注,第5天行多聚甲醛灌注,石蜡包埋,尼氏染色观察海马CA1区神经元的存活情况。结果在脑缺血再灌注后5 d,单次应用硝普钠未见明显保护作用,连续应用硝普钠7次和应用AMT和7-NI组存活率较未给药组明显增多,尼氏染色显示海马神经元细胞的存活率较对照组明显增多。结论硝普钠、7-NI和AMT均对急性脑缺血后海马神经元具有重要的保护作用。
ObjectiveTo investigate the protective effects of sodium nitroprusside（SNP） and nitric oxide synthase inhibitors（7-NI and AMT）on hippocampal neuron injury caused by cerebral ischemia/reperfusion in rats.MethodsEighty Sprague-Dawley rats with total cerebral ischemia were divided into 8 groups.According to different administration interval and approach,transient（15 minutes） brain ischemia was induced by the four-vessel occlusion（4 VO） in Sprague-Dawley rats.On the fifth day after reperfusion,the rats were perfusion;the brains were sectioned and stained with crystal violet to examine the survival of CA1 pyramidal cells in the hippocampus.ResultsThe rats treated with sodium nitroprusside for one time twenty minutes earlier before obstructing the common carotid artery did not show the protection on hippocampus neurons.The rats treated with sodium nitroprusside for seven times,7-NI and AMT had better survival rate.Crystal violet showed the rats treated with Sodium Nitroprusside for seven times,7-NI and AMT had conspicuous protection on hippocampus neurons than control group.ConclusionSNP,7-NI and AMT have neuro-protective effects on hippocampal neurons against the injury caused by cerebral ischemia/reperfusion.
出处
《中国实用神经疾病杂志》
2011年第3期8-10,共3页
Chinese Journal of Practical Nervous Diseases
关键词
硝普钠
一氧化氮合酶抑制剂
神经元
脑缺血/再灌注
脑保护
Sodium nitroprusside（SNP）
Nitric oxide synthase inhibitors
Neuron
Cerebral ischemia/reperfusion
Neuron protection
