摘要
目的探讨慢性乙型肝炎和肝炎后肝硬化患者血清基质金属蛋白酶9(MMP-9)的表达水平及其临床意义,判断MMP-9是否可以评估肝纤维化程度。方法用双抗体"夹芯"酶联免疫吸附试验(ELISA)法检测30例慢性乙型肝炎患者(肝炎组)、30例肝炎后肝硬化患者(肝硬化组)、15例健康义务献血者(健康对照组)血清MMP-9、肿瘤坏死因子α(TNF-α)、白介素6(IL-6)的表达水平,并分析肝炎组及肝硬化组患者血清MMP-9水平与TNF-α及IL-6水平的相关性。结果肝硬化组、肝炎组患者血清MMP-9、TNF-α、IL-6水平均显著高于肝炎组,且肝硬化组患者血清MMP-9水平又显著高于肝炎组,差异均有统计学意义(P<0.05)。肝炎组及肝硬化组患者血清TNF-α、IL-6表达水平与MMP-9表达水平无相关性(P>0.05);但TNF-α的表达水平与IL-6表达水平呈正相关(P<0.05)。结论慢性乙型肝炎及肝炎后肝硬化患者血清MMP-9表达升高,可作为判断肝纤维化程度的参考指标;TNF-α及IL-6对慢性乙型肝炎及肝炎后肝硬化患者血清MMP-9的表达影响小。
Objective To investigate the changes and clinical significance of serum MMP-9,and to determine whether MMP-9 can be used to assess the degree of liver cirrhosis. Methods Serum MMP-9,TNF-α and IL-6 were detected by ELISA in 30 patients with chronic hepatitis B,30 patients with liver cirrhosis and 15 heathy persons.Analyze the relationship between the expression levels of the MMP-9 with the TNF-α and IL-6. Results The serum MMP-9 levels of the patients with liver cirrhosis were significantly higher than the controls and chronic hepatitis（P0.05）;The serum TNF-α and IL-6 levels of patients with chronic hepatitis as well as liver cirrhosis were significantly higher than the heathy persons（P0.05）;There was no relationship between the expression levels of serum TNF-α and IL-6 with MMP-9 in patients with chronic hepatitis and liver cirrhosis（P0.05）.But the expression level of serum TNF-α was positively correlated with that of the serum IL-6 in patients with chronic hepatitis and liver cirrhosis（P0.05）. Conclusion The serum levels of MMP-9 was significantly inceased in chronic hepatitis B and liver cirrhosis and measurement of it can be used as diagnostic indices for liver fibrosis in chronic liver disease.TNF-α and IL-6 have little effect on expression of MMP-9 levels of the patients with chronic hepatitis and liver cirrhosis.
出处
《中国全科医学》
CAS
CSCD
北大核心
2011年第9期945-947,共3页
Chinese General Practice
关键词
基质金属蛋白酶9
肿瘤坏死因子Α
白介素6
肝炎
乙型
慢性
肝硬化
Matrix metalloproteinase 9
Tumor necrosis factor-alpha
Interleukin-6
Hepatitis B
chronic
Liver cirrhosis
