摘要
目的探讨柴胡总皂苷粗提取物对大鼠急性毒性的影响。方法采用经典的急性毒性试验方法,进行柴胡总皂苷粗提取物对大鼠的急性毒性研究。结果按含柴胡总皂苷含量计算,柴胡总皂苷粗提取物对大鼠的半数致死量(LD50)为2255.6mg·kg-1·d-1,95%可信限为2057.2～2452.9mg·kg-1·d-1。死亡大鼠急性毒性主要表现为安静怠动,继而出现步态不稳、腹卧昏睡、心率加快、呼吸急促、连续性抽搐、神经抑制而死亡。存活小鼠在14天观察期内体重增长缓慢,余未见明显异常。结论柴胡总皂苷粗提取物可致大鼠全部死亡,且其毒性比小鼠毒性小。其产生毒性的部位及毒性机制有待于深入研究。
Objective To discuss the effect of total Bupleurum saponin extracts on rat＇s acute toxicity.Methods Make the study on rat＇s acute toxicity of thetotal Bupleurum saponin extracts in accordance with classical acute toxicity test methods.Results Caculated by totalsaikosaponin,the LD50 is 2 255.6 mg·kg-·1d-1,and the 95% confidence interval is 2 057.2～2 452.9 mg·kg-·1d-1.The main acute toxicity symptoms of dead rat are negligent action,then arising from unsteady gait,abdominal lying sleeping,rapid heart rate,shortness of breath,continuous convulsions,neurological inhibition,and eventually to death.The surviving rat＇s weight gainings are slow in 14d observation period and there are no other obvious abnormalities.Conclusion The total Bupleurum saponin extracts can induce all rats to be dead,and its toxicity on rats is smaller than the mice.The toxical location and mechanism are all to be further studied.
出处
《中国药物警戒》
2010年第9期524-526,共3页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金项目(30672649)
山东省科技平台建设项目(2008GG2NS02021)
山东省科技公关关键技术研究课题(2007GG2NS02073)
山东省国际合作引智项目(L20083700336)
国家863科技攻关计划课题(2006GG2202065)
国家重点基础研究发展计划(973)中医基础理论专项资助项目(项目编号:2009CB522802)
