摘要
目的比较不同工艺路线得到的王不留行提取部位体外抑制新生血管作用。方法采用系统溶剂萃取法和按目标萃取将王不留行水提物及醇提物分别进行部位分离,得到有机萃取相,经旋蒸、冷冻干燥得冻干粉末。SRB法测定药物对人微血管内皮HMEC-1细胞增殖的影响及IC50大小;划线法测定细胞迁移率;通过测定细胞外液LDH的释放监测并比较不同提取部位的细胞毒性。结果 A4,A7,B4,B7 4个组分IC50分别为6.44±0.2,12.78±0.4,7.25±0.07及10.35±0.21;药效依次为A4>B4>B7>A7;细胞迁移距离为B4<A4<A7<B7;细胞培养液LDH活性(U.L-1)分别为758.1±19.73,1006.54±129.41,1111.11±36.97及617.45,4-75.93。结论王不留行醇提取物按系统溶剂萃取法得到的正丁醇部位是相对活性好毒性小的有效部位。
OBJECTIVE Compare antiangiogenesis effect of different extracts from Vaccaria segetalis.METHODS Vaccaria water extract and ethanol extract were isolated separately by system solvent extraction and objective extraction,keep the organic extraction phase then remove organic solvent under reduced pressure,the product was concentrated by freeze drying.Determination human microvascular endothelial cell（HMEC） proliferation and IC50 by SRB method;Scraping line method was used to observe cell migration;To monitor and compare the cytotoxicity of different extracts by measuring the release of LDH in extracellular fluid.RESULTS IC50 of A4,A7,B4,B7 corresponding to 6.44±0.2,12.78±0.4,7.25±0.07 and 10.35±0.21;which means efficacy order was A4B4B7A7;cell migration distance was A7B4B7A4;cell culture medium LDH activity was 758.1±19.73,1006.54±129.41,1111.11±36.97 and 617.45±75,93 corresponding to A4,A7,B4,B7.CONCLUSION N-butanol extract of Vaccaria alcohol extract isolated by system solvent extracton was therelative high actLvity part with low toxicity.
出处
《海峡药学》
2010年第12期34-37,共4页
Strait Pharmaceutical Journal
基金
国家自然基金项目(30772586)
