摘要
目的 探讨异丙酚对大鼠海马神经元缺氧复氧时线粒体膜通透性的影响.方法 原代培养胎鼠海马神经元,随机分为3组：对照组（C组）正常培养;模型组（M组）缺氧低糖培养2 h后复氧;异丙酚组（P组）缺氧低糖培养前加入异丙酚,终浓度20 μmol/L.各组于复氧后即刻、4、8、12和24 h（T1～5）时分别用噻唑蓝比色法测定神经元活力,流式细胞仪检测线粒体膜电位（MMP）,于T5时采用激光共聚焦显微镜观察细胞膜与线粒体膜通透性.结果 与C组相比,M组T1-5时神经元活力和MMP降低（P＜0.05）,T5时细胞膜与线粒体膜通透性增加;与M组相比,P组T1～4时神经元活力升高,T1～5时MMP增加（P＜0.05）,T5时细胞膜与线粒体膜通透性降低,完整性改善.结论 异丙酚可通过提高MMP,改善细胞膜与线粒体膜通透性,增强神经元活力,从而减轻大鼠海马神经元缺氧复氧损伤.
Objective To investigate the effects of propofol on mitochondrial membrane permeability during hypoxia/reoxygenation （H/R） in rat hippocampal neurons. Methods Primary cultured hippocampal neurons of fetal rats obtained from Wistar （17-18 days of gestation） were randomly divided into 3 groups： Ⅰ control group （group C）, Ⅱ H/R group and Ⅲ propofol ＋ H/R group. Neurons were cultured in the culture medium with combined oxygen glucose deprivation for 2 h followed by reoxygenation in group Ⅱ and Ⅲ. In group Ⅲ propofol was added to the culture medium with the final concentration of 20 μ mol/L before combined oxygen glucose deprivation.Neuronal viability was detected by MTT assay and mitochondrial membrane potential （MMP） with flow cytometry at 0, 4, 8, 12 and 24 h after reoxygenation （T1-5） and the permeability of cell membrane and mitochondrial membrane was monitored at T5 using laser confocal scanning microscope. Results The neuronal viability and MMP were significantly decreased （P 〈 0.05）, while the permeability of cell membrane and mitochondrial membrane was increased at T5 in group Ⅱ as compared to group Ⅰ . The neuronal viability at T1-4 and MMP at T1-5 were significantly increased （P 〈 0.05）, while the permeability of cell membrane and mitochondrial membrane was decreased at T5 in group Ⅲ compared to group Ⅱ . Conclusion Propofol can protect rat hippocampal neurons against H/R injury through increasing MMP, improving the cell and mitochondrial membrane permeability, and increasing the neuronal viability.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第11期1381-1383,共3页
Chinese Journal of Anesthesiology
基金
基金项目：第三批济南留学人员创业计划（03104）
关键词
二异丙酚
细胞低氧
氧
线粒体膜
通透性
海马
神经元
Propofol
Cell hypoxia
Oxygen
Mitochondrial membranes
Permeability
Hippocampus
Neurons
