摘要
目的该实验拟研究鸦胆子油乳注射液对人脑胶质瘤U251细胞生长抑制及诱导细胞凋亡的作用。方法体外培养人脑胶质瘤细胞U251,利用MTT比色法检测鸦胆子油乳注射液对于U251的增殖抑制影响;用流式细胞术(flowcytometry,FCM)分析药物处理前后U251的细胞周期变化;实时荧光定量PCR检测不同处理组中凋亡基因Bcl-2的表达。结果 MTT显示鸦胆子油乳注射液对U251细胞增殖抑制作用呈剂量依赖性,当浓度为10 g.L-1时,对U251细胞增殖的抑制率达到(75.8±2.3)%(P<0.05);流式细胞仪分析显示,用10 g.L-1鸦胆子油乳注射液培养48 h,细胞周期中G1期所占比例增高,S期占细胞周期的比例降低;实时荧光定量PCR结果显示随着鸦胆子油乳注射液的浓度增加凋亡基因Bcl-2 mRNA的表达逐渐减少。与对照组比较P<0.01。结论中药鸦胆子油乳注射液可显著抑制U251胶质瘤细胞细胞增殖及促进其凋亡。
Aim To explore the effect of brucea javanica oil emulsion on apoptosis and restraining the growth of human glioma U251.Methods Human U251 glioma cells were cultured.MTT colorimetric assay was used to measure the inhibitory effect on proliferation of brucea javanica oil emulsion for U251 golima.Flow cytometry was used to analyze the change in the cycle of U251 glioma cells.Real-time quantitative PCR was used to measure the expression of Bcl-2 in different group.Results The result of MTT showed the restrain of brucea javanica oil emulsion for U251 glioma was dose-dependent.When the consistence was 10 g·L-1,the proliferation rate was（75.8±2.3）%（P0.05）.Flowcytometry analysis showed that,with 10 g·L-1 brucea javanica oil emulsion cultured 48 h,cell cycle G1 phase proportion increased,the proportion of S phase cell cycle reduced.Real-time quantitative PCR showed that with the increae of brucea javanica oil emulsion the expression of Bcl-2 mRNA became lower and lower（P0.01）.Conclusion The brucea javanica oil emulsion can remarkably restrain the multiplication of U251 glioma and promote the cell to apoptosis.
出处
《安徽医药》
CAS
2011年第3期291-293,共3页
Anhui Medical and Pharmaceutical Journal
