摘要
目的研究麒麟菜海藻色素糖蛋白(SPG)对小鼠肝癌细胞PCNA和Bcl-2蛋白表达的影响。方法将50只皮下接种H22肝癌细胞株的小鼠随机分为5组,每组10只。高、中、低剂量SPG组分别每日经口灌胃给予SPG100、50、10mg/kg,肿瘤对照组给予等量生理盐水,连续10d。环磷酰胺组隔天腹腔注射环磷酰胺20mg/kg。取肝癌组织用MTT法测定各组肝癌细胞增殖活性,免疫组化法检测各组肝癌组织增殖细胞核抗原(PCNA)和凋亡相关蛋白Bcl-2表达水平。结果高剂量SPG组和肿瘤对照组的肝癌细胞增殖活性以吸光度表示分别为(0.711±0.028)和(1.135±0.032),差别有显著性(P<0.05)。PCNA和Bcl-2蛋白表达率在高剂量组分别为28.32%和16.78%,肿瘤对照组分别为72.78%和65.16%,差异均有显著性(P<0.05)。结论 SPG可降低小鼠肝癌细胞PCNA和Bcl-2表达水平,对肝癌细胞增殖有一定抑制作用。
Objective To investigate the effect of seaweed pigment glycoprotein（SPG） on the expression of PCNA and Bcl-2 protein in hepatocarcinoma cells in mice.Methods Fifty mice inoculated with hepatocarcinoma cells were divided into five groups,10 mice in each group.SPG was given by gavage at the dosage of 100,50 and 10 mg/kg everyday to high,medium and low dose group respectively,normal saline was given orally to tumor control group,and cyclophosphamide 20mg/kg was injected to the cylophosphamide group every the other day,all mice were sacrificed 10 days later.The proliferation activity of hepatocarcinoma cells was determined by MTT assay,and the expression of proliferating cell nuclear antigen（PCNA） and Bcl-2 protein were measured by immunohistochemical method.Results The cell proliferation activity expressed by the optical density of the high-dose group and tumor control group were 0.711 ± 0.028 and 1.135 ± 0.032 respectively（P 0.05）.The expression of PCNA and Bcl-2 protein in high-dose group was 28.32% and 16.78% respectively,and that in tumor control group was 72.78% and 65.16% respectively（P 0.05）.Conclusion The proliferation activity of hepatocarcinoma cells in mice could be reduced and the expression of PCNA and Bcl-2 protein could be inhibited by SPG.
出处
《卫生研究》
CAS
CSCD
北大核心
2011年第2期220-222,共3页
Journal of Hygiene Research
关键词
海藻色素糖蛋白
肝癌细胞
增殖活性
增殖细胞核抗原Bcl-2
seaweed pigment glycoprotein
hepatocarcinoma cell
proliferation
proliferating cell nuclear antigen
Bcl-2 protein
