摘要
以瓠瓜抗白粉病品系‘J083’和感病品系‘J73’及它们的F1代和F2代分离群体为试验材料,经接种鉴定和抗性遗传规律分析表明:瓠瓜品系‘J083’对白粉病的抗性受单隐性基因控制;从100对扩增片段长度多态性(AFLP)引物组合中获得稳定的多态性引物组合1对,即E-ATG/M-CTC;经回收、测序,特异片段全长为105 bp,并成功将其转化为序列特征性扩增区域(SCAR)标记;经连锁分析,该SCAR标记与白粉病抗性基因的连锁距离为9.6 cM,将其命名为GPDSATG/CTC75.此标记可用于瓠瓜抗白粉病品种的辅助选育.
The genetic analysis of bottle gourd [Lagenaria siceraria （Molina） Standl.] resistance to powdery mildew was evaluated with a highly resistant breeding line J083 and a highly susceptible breeding line J73 and their F1 , F2 populations. The results showed that the resistance to powdery mildew in J083 was controlled by a single recessive gene. From 100 amplified fragment length polymorphism （AFLP） primer pair combinations, a pair of stable polymorphic AFLP markers （E-ATG/M-CTC）, 105 bp in size was obtained. The AFLP fragment was then converted to a sequence-characterized amplified region （SCAR） marker, named as GPDSATC/CTC75 . This SCAR marker could be used effectively for molecular markerassisted selection （MAS） in breeding programs to develop bottle gourd cultivars resistance to powdery mildew.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2011年第2期119-124,共6页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省科技厅优先主题资助项目(2007C12003)
关键词
瓠瓜
白粉病
序列特征性扩增区域
分子标记
bottle gourd
powdery mildew
sequence-characterized amplified region
molecular marker
