摘要
目的探讨人胃癌组织中hsa-miR-124a基因启动子区的DNA甲基化状态与Rb和CDK6蛋白表达及临床病理因素的关系。方法用甲基化特异性PCR（MSP）方法检测手术切除的30例胃癌及相应癌旁组织的hsa-miR-124aDNA甲基化状态：采用免疫组织化学染色研究Rb和CDK6蛋白在胃癌组织中的表达情况。结果胃癌组织中hsa-miR-124a总体甲基化率为73．3％（22／30），明显高于癌旁组织的10．0％（3／30）（P〈0．05），其甲基化状态与肿瘤大小、分化程度、淋巴结转移、侵犯程度等临床病理因素有关（P〈0．01）。hsa-miR-124a甲基化与Rb蛋白和CDK6蛋白表达之间呈正相关（P〈0．05）。结论胃癌组织中hsa-miR-124a处于高甲基化状态，这种高甲基化可能通过增强Rh及CDK6蛋白的表达来促进胃癌的恶性增殖。
Objective To investigate the DNA methylation status of the promoter region within the coding gene hsa-miR-124a in human gastric cancer tissue, and examine its association with the expression of Rb and CDK6 protein and clinicopathological factors. Methods Methylation-specific PCR （MS-PCR） was used to detect the DNA methylation status of hsa-miR-124a in gastric cancer tissues and adjacent normal tissues from 30 patients. The expression of Rb and CDK6 protein within these tissues was examined using immunohistochemistry. Results The overall methylation rate of gastric cancer tissues was 73.3%, which was significantly higher than that in the adjacent tissues （10.0% , P〈0.05）. The overall positive rates of Rb and CDK6 protein in gastric cancer tissues were 86.7% and 80.0%, respectively. DNA methylation of hsa-miR-124a was positively correlated with the expression of Rb and CDK6 proteins. Significant associations were found between hypermethylation of hsa-miR-124a and tumor size, differentiation, lymphatic metastasis, and invasion depth （P〈0.0I）. Conclusions Hypermethy]ation of hsa-miR-124a is present in gastric cancer, and is associated with increased expression of CDK6 and Rb protein. These may be related to the proliferation and development of malignancy in the stomach.
出处
《中华胃肠外科杂志》
CAS
北大核心
2011年第2期136-139,共4页
Chinese Journal of Gastrointestinal Surgery
基金
2010南京医科大学科技发展基金重点项目（NJMUZ04）
